Rabbit Geminin Polyclonal Antibody | anti-GMNN antibody

Geminin Rabbit Polyclonal

Cat. Num. AAA769656

• Gene Names: GMNN; Gem; MGORS6
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: Geminin; Polyclonal Antibody; Geminin Rabbit Polyclonal; anti-GMNN antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 209

• Applicable Applications for anti-GMNN antibody: ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
• Immunogen: Geminin, DNA replication inhibitor
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using MBS769656 (GMNN Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer (pH9) .)

Western Blot (WB)

(human testis tissue were subjected to SDS PAGE followed by western blot with MBS769656 (GMNN antibody) at dilution of 1:300)

NCBI and Uniprot Product Information

• NCBI GI #: 354681985
• NCBI GeneID: 51053
• NCBI Accession #: NP_001238920.1
• NCBI GenBank Nucleotide #: NM_001251991.1
• Molecular Weight: 23,565 Da
• NCBI Official Full Name: geminin
• NCBI Official Synonym Full Names: geminin, DNA replication inhibitor
• NCBI Official Symbol: GMNN
• NCBI Official Synonym Symbols: Gem; MGORS6
• NCBI Protein Information: geminin
• UniProt Protein Name: Geminin
• Protein Family: Geminin
• UniProt Gene Name: GMNN
• UniProt Entry Name: GEMI_HUMAN

NCBI Description

This gene encodes a protein that plays a critical role in cell cycle regulation. The encoded protein inhibits DNA replication by binding to DNA replication factor Cdt1, preventing the incorporation of minichromosome maintenance proteins into the pre-replication complex. The encoded protein is expressed during the S and G2 phases of the cell cycle and is degraded by the anaphase-promoting complex during the metaphase-anaphase transition. Increased expression of this gene may play a role in several malignancies including colon, rectal and breast cancer. Alternatively spliced transcript variants have been observed for this gene, and two pseudogenes of this gene are located on the short arm of chromosome 16. [provided by RefSeq, Oct 2011]

Uniprot Description

Geminin: Inhibits DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle. Homotetramer. Interacts with CDT1; inhibits binding of the MCM complex to origins of replication. Interacts with IDAS; targets GMNN to the nucleus, prevents GMNN interaction with CDT1 and competes with IDAS homodimerization. Belongs to the geminin family.

Protein type: Cell cycle regulation; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus

Molecular Function: histone deacetylase binding; protein binding; transcription corepressor activity

Biological Process: cell cycle; negative regulation of cell cycle; negative regulation of DNA replication; negative regulation of transcription, DNA-dependent; organ morphogenesis; protein complex assembly