Western Blot (WB)
(Western Blot: Sample: Lane1: Porcine Brain Tissue; Lane2: Mouse Brain Tissue; Lane3: Human U-87MG Cells; Lane4: Human Hela Cells.)
Rabbit anti-Human GDP Dissociation Inhibitor 1 Polyclonal Antibody | anti-GDI1 antibody
Polyclonal Antibody to GDP Dissociation Inhibitor 1 (GDI1)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MDEEYDVIVL GTGLTECILS GIMSVNGKKV LHMDRNPYYG GESSSITPLE ELYKRFQLLE GPPESMGRGR DWNVDLIPKF LMANGQLVKM LLYTEVTRYL DFKVVEGSFV YKGGKIYKVP STETEALASN LMGMFEKRRF RKFLVFVANF DENDPKTFEG VDPQTTSMRD VYRKFDLGQD VIDFTGHALA LYRTDDYLDQ PCLETVNRIK LYSESLARYG KSPYLYPLYG LGELPQGFAR LSAIYGGTYM LNKPVDDIIM
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Lane1: Porcine Brain Tissue; Lane2: Mouse Brain Tissue; Lane3: Human U-87MG Cells; Lane4: Human Hela Cells.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Porcine Brain Tissue; Lane2: Mouse Brain Tissue; Lane3: Human U-87MG Cells; Lane4: Human Hela Cells.)
Western Blot (WB)
(Western Blot: Sample: Recombinant GDI1, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant GDI1, Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Stomach Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Stomach Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Breast Cancer Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Breast Cancer Tissue.)
Knockout Validation
(Knockout Validation: Lane 1: Wild-type U87MG cell lysate; Lane 2: GDI1 knockout U87MG cell lysate; Predicted MW: 51kd Observed MW: 60kd Primary Ab: 5ug/ml Rabbit Anti-Human GDI1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type U87MG cell lysate; Lane 2: GDI1 knockout U87MG cell lysate; Predicted MW: 51kd Observed MW: 60kd Primary Ab: 5ug/ml Rabbit Anti-Human GDI1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
NCBI and Uniprot Product Information
NCBI Description
GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, small GTP-binding proteins of the ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. GDIs slow the rate of dissociation of GDP from rab proteins and release GDP from membrane-bound rabs. GDI1 is expressed primarily in neural and sensory tissues. Mutations in GDI1 have been linked to X-linked nonspecific cognitive disability. [provided by RefSeq, Jul 2008]
Uniprot Description
GDI1: a GDP dissociation inhibitor protein expressed primarily in neural and sensory tissues. GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the Rab family, small GTP-binding proteins of the Ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. GDIs slow the rate of dissociation of GDP from Rab proteins and release GDP from membrane-bound Rabs. Mutations have been linked to X-linked nonspecific mental retardation.
Protein type: G protein regulator, misc.; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: Xq28
Cellular Component: axon; cell soma; cytoplasm; cytosol; Golgi apparatus; midbody; myelin sheath; protein complex
Molecular Function: GDP-dissociation inhibitor activity; GTPase activator activity; protein binding; Rab GDP-dissociation inhibitor activity; Rab GTPase binding
Biological Process: negative regulation of axonogenesis; negative regulation of protein targeting to membrane; positive regulation of axon extension; positive regulation of GTPase activity; protein transport; Rab protein signal transduction; regulation of small GTPase mediated signal transduction; response to calcium ion; signal transduction
Disease: Mental Retardation, X-linked 41
Research Articles on GDI1
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Product Notes
The GDI1 gdi1 (Catalog #AAA2028364) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to GDP Dissociation Inhibitor 1 (GDI1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GDP Dissociation Inhibitor 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the GDI1 gdi1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MDEEYDVIVL GTGLTECILS GIMSVNGKKV LHMDRNPYYG GESSSITPLE ELYKRFQLLE GPPESMGRGR DWNVDLIPKF LMANGQLVKM LLYTEVTRYL DFKVVEGSFV YKGGKIYKVP STETEALASN LMGMFEKRRF RKFLVFVANF DENDPKTFEG VDPQTTSMRD VYRKFDLGQD VIDFTGHALA LYRTDDYLDQ PCLETVNRIK LYSESLARYG KSPYLYPLYG LGELPQGFAR LSAIYGGTYM LNKPVDDIIM. It is sometimes possible for the material contained within the vial of "GDP Dissociation Inhibitor 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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