Rabbit anti-Human GCNT3 Polyclonal Antibody | anti-GCNT3 antibody

GCNT3, NT (GCNT3, Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3, C2GnT-mucin type, Core 2/core 4 beta-1,6-N-acetylglucosaminyltransferase) (FITC)

Cat. Num. AAA6301712

• Gene Names: GCNT3; GNTM; C24GNT; C2GNT2; C2GNTM; C2/4GnT
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GCNT3; Polyclonal Antibody; NT (GCNT3; Beta-1; 3-galactosyl-O-glycosyl-glycoprotein beta-1; 6-N-acetylglucosaminyltransferase 3; C2GnT-mucin type; Core 2/core 4 beta-1; 6-N-acetylglucosaminyltransferase) (FITC); 6-N-acetylglucosaminyltransferase; anti-GCNT3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-GCNT3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: GCNT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72-102 amino acids from the N-terminal region of human GCNT3.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GCNT3 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the GCNT3 antibody detected the GCNT3 protein (arrow).)

Related Product Information for anti-GCNT3 antibody

This gene encodes a member of the N-acetylglucosaminyltransferase family. The encoded protein is a beta-6-N-acetylglucosamine-transferase that catalyzes the formation of core 2 and core 4 O-glycans on mucin-type glycoproteins.

Product Categories/Family for anti-GCNT3 antibody

Antibodies; Enzymes; Glycosyltransferase

References

Tan, S., et al. Am. J. Respir. Cell Mol. Biol. 36(6):737-745(2007). Lamesch, P., et al. Genomics 89(3):307-315(2007). Huang, M.C., et al. Oncogene 25(23):3267-3276(2006). Beum, P.V., et al. Am. J. Respir. Cell Mol. Biol. 29(1):48-56(2003). Schwientek, T., et al. J. Biol. Chem. 274(8):4504-4512(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 4758422
• NCBI GeneID: 9245
• NCBI Accession #: NP_004742.1
• NCBI GenBank Nucleotide #: NM_004751.2
• UniProt Accession #: O95395
• Molecular Weight: 50,864 Da
• NCBI Official Full Name: beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3
• NCBI Official Synonym Full Names: glucosaminyl (N-acetyl) transferase 3, mucin type
• NCBI Official Symbol: GCNT3
• NCBI Official Synonym Symbols: GNTM; C24GNT; C2GNT2; C2GNTM; C2/4GnT
• NCBI Protein Information: beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3; hC2GnT-M; C2GnT-mucin type; beta1,6GlcNAc-transferase; beta1,6-N-acetylglucosaminyltransferase-M; core 2/core 4 beta-1,6-N-acetylglucosaminyltransferase; mucus-type co
• UniProt Protein Name: Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3
• Protein Family: Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase
• UniProt Gene Name: GCNT3
• UniProt Synonym Gene Names: C2GnT-M; hC2GnT-M; C2/4GnT
• UniProt Entry Name: GCNT3_HUMAN

NCBI Description

This gene encodes a member of the N-acetylglucosaminyltransferase family. The encoded protein is a beta-6-N-acetylglucosamine-transferase that catalyzes the formation of core 2 and core 4 O-glycans on mucin-type glycoproteins.[provided by RefSeq, Apr 2009]

Uniprot Description

GCNT3: Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development. Belongs to the glycosyltransferase 14 family.

Protein type: EC 2.4.1.150; Transferase; EC 2.4.1.102; Membrane protein, integral; Glycan Metabolism - O-glycan biosynthesis

Chromosomal Location of Human Ortholog: 15q21.3

Cellular Component: Golgi membrane; membrane; integral to membrane

Molecular Function: beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity; N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity; acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; tissue morphogenesis; O-glycan processing; immunoglobulin production in mucosal tissue; carbohydrate metabolic process; post-translational protein modification; intestinal absorption