Western Blot (WB)
(GCNT3 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the GCNT3 antibody detected the GCNT3 protein (arrow).)
Rabbit GCNT3 Polyclonal Antibody | anti-GCNT3 antibody
GCNT3 Antibody (C-term)
Western Blot (WB)
(GCNT3 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the GCNT3 antibody detected the GCNT3 protein (arrow).)
Western Blot (WB)
(GCNT3 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the GCNT3 antibody detected the GCNT3 protein (arrow).)
Immunohistochemistry (IHC)
(GCNT3 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GCNT3 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(GCNT3 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GCNT3 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(GCNT3 Antibody (C-term) flow cytometric analysis of NIH-3T3 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(GCNT3 Antibody (C-term) flow cytometric analysis of NIH-3T3 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
N-acetylglucosaminyltransferase family. The encoded protein is a
beta-6-N-acetylglucosamine-transferase that catalyzes the formation
of core 2 and core 4 O-glycans on mucin-type glycoproteins.
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Huang, M.C., et al. Oncogene 25(23):3267-3276(2006)
Beum, P.V., et al. Am. J. Respir. Cell Mol. Biol. 29(1):48-56(2003)
Schwientek, T., et al. J. Biol. Chem. 274(8):4504-4512(1999)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the N-acetylglucosaminyltransferase family. The encoded protein is a beta-6-N-acetylglucosamine-transferase that catalyzes the formation of core 2 and core 4 O-glycans on mucin-type glycoproteins.[provided by RefSeq, Apr 2009]
Uniprot Description
GCNT3: Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development. Belongs to the glycosyltransferase 14 family.
Protein type: Membrane protein, integral; EC 2.4.1.150; Glycan Metabolism - O-glycan biosynthesis; EC 2.4.1.102; Transferase
Chromosomal Location of Human Ortholog: 15q21.3
Cellular Component: Golgi membrane; membrane; integral to membrane
Molecular Function: beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity; N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity; acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity
Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; tissue morphogenesis; O-glycan processing; immunoglobulin production in mucosal tissue; carbohydrate metabolic process; post-translational protein modification; intestinal absorption
Research Articles on GCNT3
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Product Notes
The GCNT3 gcnt3 (Catalog #AAA9203729) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 390-418. The GCNT3 Antibody (C-term) reacts with Human, mouse (Predicted Reactivity: Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's GCNT3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the GCNT3 gcnt3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GCNT3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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