Western Blot (WB)
(Western blot analysis of GCLM expression in K562 cell lysate.)
Rabbit GCLM Polyclonal Antibody | anti-GCLM antibody
Anti-GCLM Rabbit pAb
Gene Names
GCLM; GLCLR
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
Affinity Chromatography
Synonyms
GCLM; Polyclonal Antibody; Anti-GCLM Rabbit pAb; GCS light chain; GLCLR; Glutamate cysteine ligase regulatory subunit; anti-GCLM antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
GCLM Antibody detects endogenous levels of total GCLM
Purity/Purification
Affinity Chromatography
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
252
Applicable Applications for anti-GCLM antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50
FC/FACS: 1:100
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50
FC/FACS: 1:100
Immunogen
A synthesized peptide derived from human GCLM
Preparation and Storage
Store at 4 degree C for short term.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.
Western Blot (WB)
(Western blot analysis of GCLM expression in K562 cell lysate.)
Western Blot (WB)
(Western blot analysis of GCLM expression in K562 cell lysate.)
Related Product Information for anti-GCLM antibody
Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31kDa
NCBI Official Full Name
glutamate--cysteine ligase regulatory subunit isoform 2
NCBI Official Synonym Full Names
glutamate-cysteine ligase modifier subunit
NCBI Official Symbol
GCLM
NCBI Official Synonym Symbols
GLCLR
NCBI Protein Information
glutamate--cysteine ligase regulatory subunit
UniProt Protein Name
Glutamate--cysteine ligase regulatory subunit
Protein Family
UniProt Gene Name
GCLM
UniProt Synonym Gene Names
GLCLR
NCBI Description
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]
Research Articles on GCLM
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Product Notes
The GCLM gclm (Catalog #AAA4751239) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GCLM Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GCLM can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:500-1:2000 IHC: 1:50-1:200 ICC/IF: 1:50-1:200 IP: 1:50 FC/FACS: 1:100. Researchers should empirically determine the suitability of the GCLM gclm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GCLM, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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