Immunohistochemistry (IHC)
(MBS9607906 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit GCLM Polyclonal Antibody | anti-GCLM antibody
GCLM Antibody
Gene Names
GCLM; GLCLR
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
GCLM; Polyclonal Antibody; GCLM Antibody; Gamma ECS regulatory subunit; Gamma-ECS regulatory subunit; Gamma-glutamylcysteine synthetase regulatory subunit; GCS light chain; GLCLR; Glutamate cysteine ligase regulatory subunit; Glutamate--cysteine ligase modifier subunit; Glutamate--cysteine ligase regulatory subunit; GSC light chain; GSH0_HUMAN; anti-GCLM antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
GCLM antibody detects endogenous levels of total GCLM
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
252
Applicable Applications for anti-GCLM antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human GCLM
Tissue Specificity
In all tissues examined. Highest levels in skeletal muscle.
Predicted Cross Reactivity
Pig, Bovine, Rabbit
Similarity
Pig (100%), Bovine (100%), Rabbit (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9607906 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9607906 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of MCF7 cell lysate, using GCLM Antibody. The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of MCF7 cell lysate, using GCLM Antibody. The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-GCLM antibody
Description: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.
Subunit Structure: Heterodimer of a catalytic heavy chain and a regulatory light chain.
Similarity: Belongs to the aldo/keto reductase family. Glutamate--cysteine ligase light chain subfamily.
Subunit Structure: Heterodimer of a catalytic heavy chain and a regulatory light chain.
Similarity: Belongs to the aldo/keto reductase family. Glutamate--cysteine ligase light chain subfamily.
References
Li L, Sun HY, Liu W, Zhao HY, Shao ML; Journal: Food Chem Toxicol. Silymarin protects against acrylamide-induced neurotoxicity via Nrf2 signalling in PC12 cells. Zou Y, Wang J, Peng J, Wei H; Journal: Oxid Med Cell Longev. Oregano Essential Oil Induces SOD1 and GSH Expression through Nrf2 Activation and Alleviates Hydrogen Peroxide-Induced Oxidative Damage in IPEC-J2 Cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 30 kDa
Predicted: 31 kDa
Predicted: 31 kDa
NCBI Official Full Name
glutamate--cysteine ligase regulatory subunit isoform 2
NCBI Official Synonym Full Names
glutamate-cysteine ligase modifier subunit
NCBI Official Symbol
GCLM
NCBI Official Synonym Symbols
GLCLR
NCBI Protein Information
glutamate--cysteine ligase regulatory subunit
UniProt Protein Name
Glutamate--cysteine ligase regulatory subunit
Protein Family
UniProt Gene Name
GCLM
UniProt Synonym Gene Names
GLCLR
NCBI Description
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]
Research Articles on GCLM
Similar Products
Product Notes
The GCLM gclm (Catalog #AAA9607906) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GCLM Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GCLM can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the GCLM gclm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GCLM, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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