Rabbit anti-Human, Mouse GCAT Polyclonal Antibody | anti-GCAT antibody

GCAT (Glycine Acetyltransferase, AKB Ligase, Aminoacetone Synthetase, 2-amino-3-ketobutyrate Coenzyme A Ligase Mitochondrial, KBL) (MaxLight 650)

Cat. Num. AAA6477632

• Gene Names: GCAT; KBL
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: GCAT; Polyclonal Antibody; GCAT (Glycine Acetyltransferase; AKB Ligase; Aminoacetone Synthetase; 2-amino-3-ketobutyrate Coenzyme A Ligase Mitochondrial; KBL) (MaxLight 650); EC=2.3.1.29; anti-GCAT antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GCAT. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-GCAT antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human GCAT (KLH).
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GCAT antibody (Center) in mouse liver tissue lysates (35ug/lane). GCAT (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain reacted with GCAT Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of HepG2 cells using GCAT Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot analysis of GCAT (arrow) using rabbit polyclonal GCAT Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GCAT gene.)

Product Categories/Family for anti-GCAT antibody

Antibodies; Enzymes; Acetyltransferase

References

1. Edgar, A.J., Polak, J.M.Eur. J. Biochem. 267(6), 1805-1812 (2000). 2. Tressel, T., Thompson, R., J. Biol. Chem. 261(35), 16428-16437 (1986).

NCBI and Uniprot Product Information

• NCBI GI #: 7657118
• NCBI GeneID: 23464
• NCBI Accession #: NP_055106
• NCBI GenBank Nucleotide #: NM_014291.3
• UniProt Accession #: O75600; Q6ZWF1; Q96CA9; E2QC23
• Molecular Weight: 45.0kDa (419aa)
• NCBI Official Full Name: 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial isoform 2
• NCBI Official Synonym Full Names: glycine C-acetyltransferase
• NCBI Official Symbol: GCAT
• NCBI Official Synonym Symbols: KBL
• NCBI Protein Information: 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial
• UniProt Protein Name: 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial
• UniProt Gene Name: GCAT
• UniProt Synonym Gene Names: KBL; AKB ligase
• UniProt Entry Name: KBL_HUMAN

NCBI Description

The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. This gene encodes the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The encoded enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 14. [provided by RefSeq, Jan 2010]

Uniprot Description

GCAT: The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. This gene encodes the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The encoded enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 14. [provided by RefSeq, Jan 2010]

Protein type: Amino Acid Metabolism - glycine, serine and threonine; Mitochondrial; Transferase; EC 2.3.1.29

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: nucleoplasm; mitochondrion

Molecular Function: glycine C-acetyltransferase activity; pyridoxal phosphate binding

Biological Process: amino acid metabolic process; biosynthetic process; threonine catabolic process to pyruvate