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Western Blot (WB) (All lanes : Anti-GBGT1 Antibody (Center) at 1:2000 dilutionLane 1: Jurkat whole cell lysateLane 2: Hela whole cell lysateLane 3: SK-OV-3 whole cell lysateLane 4: A2780 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit anti-Human GBGT1 Polyclonal Antibody | anti-GBGT1 antibody

GBGT1 Antibody (Center)

Gene Names
GBGT1; FS; A3GALNT; UNQ2513
Reactivity
Human
Applications
Western Blot, ELISA
Synonyms
GBGT1; Polyclonal Antibody; GBGT1 Antibody (Center); Globoside alpha-1;3-N-acetylgalactosaminyltransferase 1; 2.4.1.-; Forssman glycolipid synthase-like protein; anti-GBGT1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Ig
Specificity
This GBGT1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 154-185 amino acids from the Central region of human GBGT1.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
294
Applicable Applications for anti-GBGT1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB:~~1:2000
Subtitle
Purified Rabbit Polyclonal Antibody (Pab)
Cellular Location
Golgi apparatus membrane; Single-pass type II membrane protein.
Tissue Location
Widely expressed. Expressed at higher level in placenta, ovary and peripheral blood leukocyte, whereas it is weakly expressed in liver, thymus, and testis.
Antigen Type
Synthesized Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-GBGT1 Antibody (Center) at 1:2000 dilutionLane 1: Jurkat whole cell lysateLane 2: Hela whole cell lysateLane 3: SK-OV-3 whole cell lysateLane 4: A2780 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (All lanes : Anti-GBGT1 Antibody (Center) at 1:2000 dilutionLane 1: Jurkat whole cell lysateLane 2: Hela whole cell lysateLane 3: SK-OV-3 whole cell lysateLane 4: A2780 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-GBGT1 antibody
Catalyzes the formation of some glycolipid via the addition of N-acetylgalactosamine (GalNAc) in alpha-1,3-linkage to some substrate. Glycolipids probably serve for adherence of some pathogens.
Product Categories/Family for anti-GBGT1 antibody
References
Xu H.,et al.J. Biol. Chem. 274:29390-29398 (1999). Clark H.F.,et al.Genome Res. 13:2265-2270 (2003). Ota T.,et al.Nat. Genet. 36:40-45 (2004). Humphray S.J.,et al.Nature 429:369-374 (2004). Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
40127 Da
NCBI Official Full Name
globoside alpha-1,3-N-acetylgalactosaminyltransferase 1 isoform 2
NCBI Official Synonym Full Names
globoside alpha-1,3-N-acetylgalactosaminyltransferase 1
NCBI Official Symbol
GBGT1
NCBI Official Synonym Symbols
FS; A3GALNT; UNQ2513
NCBI Protein Information
globoside alpha-1,3-N-acetylgalactosaminyltransferase 1
UniProt Protein Name
Globoside alpha-1,3-N-acetylgalactosaminyltransferase 1
UniProt Gene Name
GBGT1
UniProt Entry Name
GBGT1_HUMAN

NCBI Description

This gene encodes a glycosyltransferase that plays a role in the synthesis of Forssman glycolipid (FG), a member of the globoseries glycolipid family. Glycolipids such as FG form attachment sites for the binding of pathogens to cells; expression of this protein may determine host tropism to microorganisms. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]

Uniprot Description

GBGT1: Catalyzes the formation of some glycolipid via the addition of N-acetylgalactosamine (GalNAc) in alpha-1,3-linkage to some substrate. Glycolipids probably serve for adherence of some pathogens. Belongs to the glycosyltransferase 6 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; EC 2.4.1.88; Glycan Metabolism - glycosphingolipid biosynthesis - globo series; Membrane protein, integral

Chromosomal Location of Human Ortholog: 9q34.13-q34.3

Cellular Component: Golgi membrane; integral to membrane

Molecular Function: globoside alpha-N-acetylgalactosaminyltransferase activity; metal ion binding

Biological Process: glycolipid biosynthetic process; protein amino acid glycosylation

Research Articles on GBGT1

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Product Notes

The GBGT1 gbgt1 (Catalog #AAA9217181) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GBGT1 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GBGT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB:~~1:2000. Researchers should empirically determine the suitability of the GBGT1 gbgt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GBGT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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