Rabbit GBF1 Polyclonal Antibody | anti-GBF1 antibody
Phospho-GBF1 (Ser292/Ser297) Antibody
Gene Names
GBF1; ARF1GEF
Reactivity
Human, Mouse, Rat
Applications
ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
GBF1; Polyclonal Antibody; Phospho-GBF1 (Ser292/Ser297) Antibody; ARF1GEF; BFA resistant GEF 1; BFA-resistant GEF 1; FLJ21263; FLJ21500; GBF1_HUMAN; golgi brefeldin A resistant guanine nucleotide exchange factor 1; Golgi specific brefeldin A resistance factor 1; Golgi specific Brefeldin A-resistance factor; Golgi-specific brefeldin A-resistance guanine nucleotide exchange; Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1; KIAA0248; MGC134877; MGC134878; anti-GBF1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-GBF1 (Ser292/Ser297) Antibody detects endogenous levels of GBF1 only when phosphorylated at Ser292/Ser297.
Tissue Specificity: Ubiquitous.
Tissue Specificity: Ubiquitous.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-GBF1 antibody
Peptide ELISA (EIA)
Application Notes
Peptide ELISA: 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human GBF1 around the phosphorylation site of Ser292/Ser297.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
AMPK-mediated phosphorylation at Thr-1337 is induced by 2-deoxyglucose (2-DG) and AICA ribonucleotide, and occurs during mitosis leading to membrane disassociation and inactivation of ARF1 during mitosis.
Subunit Structure
Can form homodimers and probably homotetramers. Interacts with COPG1; the interaction is independent on ARF1 activation. Interacts with ARF1, ARF3, ARF4 and ARF5. Interacts with RAB1B (GTP-bound form); required for GBF1 membrane association. Interacts with GGA1, GGA2 and GGA3. Interacts with USO1. Interacts (via SEC7 domain) with PNPLA2 (via C-terminus); the interaction is direct. Interacts with ARMH3. (Microbial infection) Interacts with poliovirus protein 3A.
Similarity
The DCB (dimerization and cyclophiln-binding) and HUS (homology upstream of Sec7) domains are necessary for dimerization. The DCB domain is proposed to support constitutive homodimerization; the HUS domain interacts with the DCB domain which may occur intramolecular or intermolecuar (By similarity).
Subcellular Location
Golgi apparatus>cis-Golgi network. Endoplasmic reticulum-Golgi intermediate compartment. Golgi apparatus>trans-Golgi network. Golgi apparatus. Cytoplasm. Lipid droplet. Membrane>Peripheral membrane protein.
Note: Cycles rapidly on and off early Golgi membranes (PubMed:15616190). Stabilized on membranes when complexed with ARF1-GDP and is released from both ARF1 and membranes after it catalyzes GDP displacement and ARF1 binds GTP. Continuous cycles of recruitment and dissociation of GBF1 to membranes are required for sustained ARF activation and COP I recruitment (PubMed:15813748). In neutrophils is translocated from the Golgi to the leading edge upon GPCR stimulation (PubMed:22573891). Localization to lipid droplets is questionable (PubMed:22185782).
Note: Cycles rapidly on and off early Golgi membranes (PubMed:15616190). Stabilized on membranes when complexed with ARF1-GDP and is released from both ARF1 and membranes after it catalyzes GDP displacement and ARF1 binds GTP. Continuous cycles of recruitment and dissociation of GBF1 to membranes are required for sustained ARF activation and COP I recruitment (PubMed:15813748). In neutrophils is translocated from the Golgi to the leading edge upon GPCR stimulation (PubMed:22573891). Localization to lipid droplets is questionable (PubMed:22185782).
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Related Product Information for anti-GBF1 antibody
Guanine-nucleotide exchange factor (GEF) for members of the Arf family of small GTPases involved in trafficking in the early secretory pathway; its GEF activity initiates the coating of nascent vesicles via the localized generation of activated ARFs through replacement of GDP with GTP. Recruitment to cis-Golgi membranes requires membrane association of Arf-GDP and can be regulated by ARF1, ARF3, ARF4 and ARF5. Involved in the recruitment of the COPI coat complex to the endoplasmic reticulum exit sites (ERES), and the endoplasmic reticulum-Golgi intermediate (ERGIC) and cis-Golgi compartments which implicates ARF1 activation. Involved in COPI vesicle-dependent retrograde transport from the ERGIC and cis-Golgi compartments to the endoplasmic reticulum (ER). Involved in the trans-Golgi network recruitment of GGA1, GGA2, GGA3, BIG1, BIG2, and the AP-1 adapter protein complex related to chlathrin-dependent transport; the function requires its GEF activity (probably at least in part on ARF4 and ARF5). Has GEF activity towards ARF1. Has in vitro GEF activity towards ARF5 (By similarity). Involved in the processing of PSAP. Required for the assembly of the Golgi apparatus. The AMPK-phosphorylated form is involved in Golgi disassembly during mitotis and under stress conditions. May be involved in the COPI vesicle-dependent recruitment of PNPLA2 to lipid droplets; however, this function is under debate. In neutrophils, involved in G protein-coupled receptor (GPCR)-mediated chemotaxis und superoxide production. Proposed to be recruited by phosphatidylinositol-phosphates generated upon GPCR stimulation to the leading edge where it recruits and activates ARF1, and is involved in recruitment of GIT2 and the NADpHoxidase complex.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
206,446 Da
NCBI Official Full Name
Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform 2
NCBI Official Synonym Full Names
golgi brefeldin A resistant guanine nucleotide exchange factor 1
NCBI Official Symbol
GBF1
NCBI Official Synonym Symbols
ARF1GEF
NCBI Protein Information
Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1; BFA-resistant GEF 1
UniProt Protein Name
Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1
Protein Family
UniProt Gene Name
GBF1
UniProt Synonym Gene Names
KIAA0248; BFA-resistant GEF 1
UniProt Entry Name
GBF1_HUMAN
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Product Notes
The GBF1 gbf1 (Catalog #AAA9612633) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-GBF1 (Ser292/Ser297) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GBF1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA). Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the GBF1 gbf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GBF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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