Rabbit Gbeta5 Polyclonal Antibody | anti-Gbeta5 antibody

Gbeta5 Rabbit pAb

Cat. Num. AAA4754284

• Gene Names: GNB5; GB5
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: Gbeta5; Polyclonal Antibody; Gbeta5 Rabbit pAb; guanine nucleotide binding protein (G protein); beta 5; anti-Gbeta5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Widely expressed.
• Purity/Purification: Affinity purified
• Form/Format: pH7.4 PBS, 0.05% NaN3, 40% Glycerol

• Applicable Applications for anti-Gbeta5 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:200-1:1000; ELISA: 1:1000-1:2000; IHC: 1:25-1:100
• Immunogen: Fusion protein of human GNB5
• Conjugation: Unconjugated
• Molecular Function: Enhances GTPase-activating protein (GAP) activity of regulator of G protein signaling (RGS) proteins, hence involved in the termination of the signaling initiated by the G protein coupled receptors (GPCRs) by accelerating the GTP hydrolysis on the G-alpha subunits, thereby promoting their inactivation (Probable). Increases RGS9 GTPase-activating protein (GAP) activity, hence contributes to the deactivation of G protein signaling initiated by D2 dopamine receptors (PubMed:27677260). May play an important role in neuronal signaling, including in the parasympathetic, but not sympathetic, control of heart rate.
• Modification: Unmodified
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Gel: 15%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Human fetal brain tissue, mouse heart tissue, Primary antibody: 120653(GNB5 Antibody) at dilution 1/550, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 second)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human brain tissue using 120653(GNB5 Antibody) at dilution 1/20. (Original magnification: ×200))

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 120653(GNB5 Antibody) at dilution 1/20. (Original magnification: ×200))

Related Product Information for anti-Gbeta5 antibody

Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. Alternatively spliced transcript variants encoding different isoforms exist.

Product Categories/Family for anti-Gbeta5 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 5729852
• NCBI GeneID: 10681
• NCBI Accession #: NP_006569.1
• NCBI GenBank Nucleotide #: NM_006578.3
• UniProt Accession #: O14775; Q9HAU9; Q9UFT3
• Molecular Weight: 43,566 Da
• NCBI Official Full Name: guanine nucleotide-binding protein subunit beta-5 isoform a
• NCBI Official Synonym Full Names: guanine nucleotide binding protein (G protein), beta 5
• NCBI Official Symbol: GNB5
• NCBI Official Synonym Symbols: GB5
• NCBI Protein Information: guanine nucleotide-binding protein subunit beta-5; gbeta5; transducin beta chain 5; G protein, beta-5 subunit; G protein, beta subunit 5L; guanine nucleotide-binding protein, beta subunit 5L
• UniProt Protein Name: Guanine nucleotide-binding protein subunit beta-5
• UniProt Gene Name: GNB5
• UniProt Entry Name: GBB5_HUMAN

NCBI Description

Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. Alternatively spliced transcript variants encoding different isoforms exist. [provided by RefSeq, Jul 2008]

Uniprot Description

G-beta 5: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction. Belongs to the WD repeat G protein beta family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein, heterotrimeric beta

Chromosomal Location of Human Ortholog: 15q21.2

Cellular Component: plasma membrane; nucleus

Molecular Function: GTPase activity; G-protein gamma-subunit binding; protein binding; signal transducer activity; chaperone binding

Biological Process: rhodopsin mediated signaling; phototransduction, visible light; regulation of rhodopsin mediated signaling; metabolic process; signal transduction