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Immunohistochemistry (IHC) ((3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

Goat APOC1 Polyclonal Antibody | anti-APOC1 antibody

Goat anti-APOC1 Antibody

Gene Names
APOC1; Apo-CI; ApoC-I; apo-CIB; apoC-IB
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
APOC1; Polyclonal Antibody; Goat anti-APOC1 Antibody; apolipoprotein C-I; APOC1 antibody; apolipoprotein C-I antibody; anti-APOC1 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
ETFQKVKEKLKIDS
Sequence Length
83
Applicable Applications for anti-APOC1 antibody
Peptide ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Immunohistochemistry: In paraffin embedded Human Liver shows spotty staining in the cytoplasm of hepatocytes. Recommended concentration, 3-5ug/ml.
Western Blot: Preliminary experiments gave bands at 30kDa and 37kDa band in Human Liver lysates after 0.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 9.3kDa according to NP_001636.1. The detected bands were successfully blocked by incubation with the immunizing peptide.
Immunogen
Peptide with sequence C-ETFQKVKEKLKIDS, from the C Terminus of the protein sequence according to NP_001636.1.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

((3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

Immunohistochemistry (IHC) ((3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
341
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
9,332 Da
NCBI Official Full Name
apolipoprotein C-I
NCBI Official Synonym Full Names
apolipoprotein C1
NCBI Official Symbol
APOC1
NCBI Official Synonym Symbols
Apo-CI; ApoC-I; apo-CIB; apoC-IB
NCBI Protein Information
apolipoprotein C-I
UniProt Protein Name
Apolipoprotein C-I
Protein Family
UniProt Gene Name
APOC1
UniProt Synonym Gene Names
Apo-CI; ApoC-I
UniProt Entry Name
APOC1_HUMAN

NCBI Description

This gene encodes a member of the apolipoprotein C1 family. This gene is expressed primarily in the liver, and it is activated when monocytes differentiate into macrophages. The encoded protein plays a central role in high density lipoprotein (HDL) and very low density lipoprotein (VLDL) metabolism. This protein has also been shown to inhibit cholesteryl ester transfer protein in plasma. A pseudogene of this gene is located 4 kb downstream in the same orientation, on the same chromosome. This gene is mapped to chromosome 19, where it resides within a apolipoprotein gene cluster. [provided by RefSeq, Mar 2016]

Uniprot Description

APOC1: Appears to modulate the interaction of APOE with beta- migrating VLDL and inhibit binding of beta-VLDL to the LDL receptor-related protein. Binds free fatty acids and reduces their intracellular esterification. Belongs to the apolipoprotein C1 family.

Protein type: Lipid-binding; Inhibitor; Endoplasmic reticulum; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: chylomicron; endoplasmic reticulum

Molecular Function: fatty acid binding; lipase inhibitor activity; phosphatidylcholine binding; phospholipase inhibitor activity

Biological Process: cholesterol efflux; cholesterol metabolic process; lipid metabolic process; lipoprotein metabolic process; negative regulation of cholesterol transport; negative regulation of fatty acid biosynthetic process; negative regulation of lipid catabolic process; negative regulation of lipid metabolic process; negative regulation of lipoprotein lipase activity; negative regulation of receptor-mediated endocytosis; phospholipid efflux; positive regulation of catalytic activity; regulation of cholesterol transport; triacylglycerol metabolic process

Research Articles on APOC1

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Product Notes

The APOC1 apoc1 (Catalog #AAA420037) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-APOC1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's APOC1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 8000. Immunohistochemistry: In paraffin embedded Human Liver shows spotty staining in the cytoplasm of hepatocytes. Recommended concentration, 3-5ug/ml. Western Blot: Preliminary experiments gave bands at 30kDa and 37kDa band in Human Liver lysates after 0.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 9.3kDa according to NP_001636.1. The detected bands were successfully blocked by incubation with the immunizing peptide. Researchers should empirically determine the suitability of the APOC1 apoc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ETFQKVKEKL KIDS. It is sometimes possible for the material contained within the vial of "APOC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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