Rabbit anti-Human GATA1 Polyclonal Antibody | anti-GATA1 antibody

GATA1 (GATA Binding Factor 1, GATA Binding Protein 1)

Cat. Num. AAA613307

• Gene Names: GATA1; GF1; GF-1; NFE1; XLTT; ERYF1; GATA-1
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity chromatography.
• Synonyms: GATA1; Polyclonal Antibody; GATA1 (GATA Binding Factor 1; GATA Binding Protein 1); Anti -GATA1 (GATA Binding Factor 1; anti-GATA1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of total GATA-1 protein.
• Purity/Purification: Affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

• Applicable Applications for anti-GATA1 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blot and Immunoprecipitation.; Dilution: Western Blot: 1:1000; Immunoprecipitation: 1:50
• Immunogen: Synthetic peptide (Blue Carrier-coupled) corresponding to residues surrounding Pro401 of human GATA-1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of total lysates from K562 and HEL cells using MBS613307)

Related Product Information for anti-GATA1 antibody

GATA proteins comprise a group of transcription factors that are related by the presence of conserved zinc finger DNA binding domains that bind directly to the nucleotide sequence core element GATA (1-3). There are six vertebrate GATA proteins, designated GATA-1 to GATA-6 (3). GATA-1 is the founding member of the GATA family and is required for erythroid and megakaryocytic cell development (4,5). Mutations in GATA-1 have been linked to many human diseases, including acute megakaryoblastic leukemia in Down syndrome children (DS-AMKL), X-linked thrombocytopenia, and gray platelet syndrome (6-9).

Product Categories/Family for anti-GATA1 antibody

Antibodies; Abs to Transcription Factors; Zinc (Ring) Finger

NCBI and Uniprot Product Information

• NCBI GI #: 14602571
• NCBI GeneID: 2623
• UniProt Accession #: P15976; Q96GB8
• Molecular Weight: 42,751 Da
• NCBI Official Full Name: GATA1 protein
• NCBI Official Synonym Full Names: GATA binding protein 1 (globin transcription factor 1)
• NCBI Official Symbol: GATA1
• NCBI Official Synonym Symbols: GF1; GF-1; NFE1; XLTT; ERYF1; GATA-1
• NCBI Protein Information: erythroid transcription factor; OTTHUMP00000023259; OTTHUMP00000023260; GATA-binding factor 1; NF-E1 DNA-binding protein; transcription factor GATA1; globin transcription factor 1; erythroid transcription factor 1
• UniProt Protein Name: Erythroid transcription factor
• Protein Family: GATA transcription factor
• UniProt Gene Name: GATA1
• UniProt Synonym Gene Names: ERYF1; GF1
• UniProt Entry Name: GATA1_HUMAN

NCBI Description

This gene encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. [provided by RefSeq]

Uniprot Description

GATA1: Transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells. May form homodimers or heterodimers with other isoforms. Interacts (via the N-terminal zinc finger) with ZFPM1. Interacts with GFI1B. Interacts with PIAS4; the interaction enhances sumoylation and represses the transactivational activity in a sumoylation-independent manner. Interacts with LMCD1. Interacts with CREBBP; the interaction stimulates acetylation and transcriptional activity in vivo. Interacts with EP300. Erythrocytes. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: nucleoplasm; transcription factor complex; transcriptional repressor complex; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; chromatin DNA binding; sequence-specific DNA binding; p53 binding; transcription factor activity; DNA bending activity

Biological Process: transcription from RNA polymerase II promoter; erythrocyte development; embryonic hemopoiesis; in utero embryonic development; megakaryocyte differentiation; positive regulation of erythrocyte differentiation; eosinophil differentiation; male gonad development; negative regulation of transcription from RNA polymerase II promoter; negative regulation of bone mineralization; negative regulation of cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; cell-cell signaling; positive regulation of osteoblast proliferation; erythrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; platelet formation; blood coagulation; basophil differentiation; negative regulation of apoptosis

Disease: Thrombocytopenia With Beta-thalassemia, X-linked; Down Syndrome; Anemia, X-linked, With Or Without Neutropenia And/or Platelet Abnormalities; Thrombocytopenia, X-linked, With Or Without Dyserythropoietic Anemia