Rabbit GATA1 Polyclonal Antibody | anti-GATA1 antibody

GATA1 (Erythroid Transcription Factor, Eryf1, GATA-binding Factor 1, GATA-1, GF-1, NF-E1 DNA-binding Protein, ERYF1, GF1)

Cat. Num. AAA624536

• Gene Names: GATA1; GF1; GF-1; NFE1; XLTT; ERYF1; GATA-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: GATA1; Polyclonal Antibody; GATA1 (Erythroid Transcription Factor; Eryf1; GATA-binding Factor 1; GATA-1; GF-1; NF-E1 DNA-binding Protein; ERYF1; GF1); Anti -GATA1 (Erythroid Transcription Factor; anti-GATA1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GATA1. Species Cross-Reactivity: mouse, rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-GATA1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, Western Blot, and Immunohistochemistry.; Dilution: Immunofluorescence: 1:100-1:200; Western Blot: 1:500-1:1,000; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic non-phosphopeptide corresponding to human GATA-1 around the phosphorylation site of serine 142 (R-L-SP-P-D).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-GATA1 antibody

Globin transcription factor 1 (GATA-1) is a transcription activator belonging to the GATA family of transcription factors with importance in cell growth. GATA-1 is a Cys2-Cys2 zinc finger DNA binding protein commonly found in erthroid (red blood cell) and emagkaryotic (platelet producing cell) and it is critical in the development process of both. It binds to the consensus sequence of [AT]GATA[AG] within regulatory regions of globin genes and genes in erythroid cells, leading to transcription of alpha spectrin structural protein necessary for red blood cell's shape. In human, GATA-1 can enhance transcription rate up to 100 times. Mutation to GATA-1 encoding gene has been implicated to X-linked dyserythropoietic anemia, thrombocytopenia, Down's syndrome in children, congenital transient myeloproliferative syndrome, and acute megakaryocytic leukemia.

Product Categories/Family for anti-GATA1 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 14602571
• NCBI GeneID: 2623
• UniProt Accession #: P15976; Q96GB8
• Molecular Weight: 42,751 Da
• NCBI Official Full Name: GATA1 protein
• NCBI Official Synonym Full Names: GATA binding protein 1 (globin transcription factor 1)
• NCBI Official Symbol: GATA1
• NCBI Official Synonym Symbols: GF1; GF-1; NFE1; XLTT; ERYF1; GATA-1
• NCBI Protein Information: erythroid transcription factor; OTTHUMP00000023259; OTTHUMP00000023260; GATA-binding factor 1; NF-E1 DNA-binding protein; transcription factor GATA1; globin transcription factor 1; erythroid transcription factor 1
• UniProt Protein Name: Erythroid transcription factor
• Protein Family: GATA transcription factor
• UniProt Gene Name: GATA1
• UniProt Synonym Gene Names: ERYF1; GF1
• UniProt Entry Name: GATA1_HUMAN

NCBI Description

This gene encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. [provided by RefSeq]

Uniprot Description

GATA1: Transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells. May form homodimers or heterodimers with other isoforms. Interacts (via the N-terminal zinc finger) with ZFPM1. Interacts with GFI1B. Interacts with PIAS4; the interaction enhances sumoylation and represses the transactivational activity in a sumoylation-independent manner. Interacts with LMCD1. Interacts with CREBBP; the interaction stimulates acetylation and transcriptional activity in vivo. Interacts with EP300. Erythrocytes. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: nucleoplasm; transcription factor complex; transcriptional repressor complex; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; sequence-specific DNA binding; p53 binding; chromatin DNA binding; DNA bending activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; erythrocyte development; in utero embryonic development; megakaryocyte differentiation; embryonic hemopoiesis; positive regulation of erythrocyte differentiation; eosinophil differentiation; male gonad development; negative regulation of transcription from RNA polymerase II promoter; negative regulation of bone mineralization; negative regulation of cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; cell-cell signaling; positive regulation of osteoblast proliferation; erythrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; platelet formation; blood coagulation; basophil differentiation; negative regulation of apoptosis

Disease: Thrombocytopenia With Beta-thalassemia, X-linked; Down Syndrome; Anemia, X-linked, With Or Without Neutropenia And/or Platelet Abnormalities; Thrombocytopenia, X-linked, With Or Without Dyserythropoietic Anemia