Rabbit anti-Human, Mouse GAS1 Polyclonal Antibody | anti-GAS1 antibody

GAS1, NT (GAS1, Growth arrest-specific protein 1) (Biotin)

Cat. Num. AAA6301260

• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: GAS1; Polyclonal Antibody; NT (GAS1; Growth arrest-specific protein 1) (Biotin); Growth arrest-specific protein 1; anti-GAS1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-GAS1 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; IF: 1:10-50; IHC: 1:50-100; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: GAS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-136 amino acids from the N-terminal region of human GAS1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GAS1 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the GAS1 antibody detected the GAS1 protein (arrow).)

Immunohistochemistry (IHC)

(GAS1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GAS1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of GAS1 Antibody (N-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(GAS1 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-GAS1 antibody

Growth arrest-specific 1 plays a role in growth suppression. GAS1 blocks entry to S phase and prevents cycling of normal and transformed cells. Gas1 is a putative tumor suppressor gene.

Product Categories/Family for anti-GAS1 antibody

Antibodies; Proteins

References

Ribeiro, L.A., et al. Am. J. Med. Genet. A 152A (7), 1688-1694 (2010): Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010): Rittie, L., et al. Aging Cell 8(6):738-751(2009). Zhao, L., et al. J. Biol. Chem. 284(39):26273-26285(2009). Dominguez-Monzon, G., et al. Int. J. Dev. Neurosci. 27(4):305-313(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 167466169
• NCBI GeneID: 2619
• NCBI Accession #: NP_002039
• NCBI GenBank Nucleotide #: NM_002048
• UniProt Accession #: P54826; Q6B086; B9EGM4
• Molecular Weight: 35,693 Da
• NCBI Official Full Name: growth arrest-specific protein 1
• NCBI Official Synonym Full Names: growth arrest-specific 1
• NCBI Official Symbol: GAS1
• NCBI Protein Information: growth arrest-specific protein 1; GAS-1; Growth arrest-specific gene-1
• UniProt Protein Name: Growth arrest-specific protein 1
• Protein Family: Germacrene A synthase
• UniProt Gene Name: GAS1
• UniProt Synonym Gene Names: GAS-1
• UniProt Entry Name: GAS1_HUMAN

Uniprot Description

GAS1: Specific growth arrest protein involved in growth suppression. Blocks entry to S phase. Prevents cycling of normal and transformed cells.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 9q21.3-q22

Cellular Component: anchored to plasma membrane; plasma membrane; integral to membrane

Molecular Function: protein binding

Biological Process: developmental growth; axon guidance; cell fate commitment; camera-type eye development; outer ear morphogenesis; programmed cell death; cerebellum morphogenesis; palate development; embryonic cranial skeleton morphogenesis; middle ear morphogenesis; odontogenesis; regulation of apoptosis; negative regulation of smoothened signaling pathway; positive regulation of mesenchymal cell proliferation; positive regulation of smoothened signaling pathway; negative regulation of mitotic cell cycle; embryonic digit morphogenesis; negative regulation of cell growth; cell cycle arrest; positive regulation of epithelial cell proliferation; dorsoventral neural tube patterning; regulation of smoothened signaling pathway; negative regulation of epithelial cell proliferation; eye morphogenesis