Mouse MAP Kinase-Activated Protein Kinase 2 (MAPKAPK2) ELISA Kit | MAPKAPK2 elisa kit

Mouse MAP Kinase-Activated Protein Kinase 2 (MAPKAPK2) ELISA Kit

Cat. Num. AAA9717737

• Gene Names: Mapkapk2; MK2; MK-2; Rps6kc1; AA960234; MAPKAP-K2
• Reactivity: Mouse
• Synonyms: MAP Kinase-Activated Protein Kinase 2 (MAPKAPK2); Mouse MAP Kinase-Activated Protein Kinase 2 (MAPKAPK2) ELISA Kit; MAPKAPK2; MK2; MAPKAPK2 elisa kit

• Reactivity: Mouse
• Sequence Length: 386

• Assay Type: Sandwich
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MAPKAPK2 elisa kit

Principle of the Assay: Mouse MAP kinase-activated protein kinase 2 (MAPKAPK2) ELISA Kit employs a two-site sandwich ELISA to quantitate MAPKAPK2 in samples. An antibody specific for MAPKAPK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MAPKAPK2 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Mouse MAPKAPK2 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of MAPKAPK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background/Introduction: Increased activation of MAPKAP2 is responsible for elevated and posttranscriptionally regulated TNF-alpha protein expression in psoriatic skin. MAPKAP kinase-2 is directly responsible for Cdc25B/C phosphorylation and 14-3-3 binding in vitro and in response to UV-induced DNA damage.

NCBI and Uniprot Product Information

• NCBI GI #: 45544580
• NCBI GeneID: 17164
• NCBI Accession #: NP_032577.1
• NCBI GenBank Nucleotide #: NP_032577.1
• UniProt Accession #: P49138
• NCBI Official Full Name: MAP kinase-activated protein kinase 2
• NCBI Official Synonym Full Names: MAP kinase-activated protein kinase 2
• NCBI Official Symbol: Mapkapk2
• NCBI Official Synonym Symbols: MK2; MK-2; Rps6kc1; AA960234; MAPKAP-K2
• NCBI Protein Information: MAP kinase-activated protein kinase 2
• UniProt Protein Name: MAP kinase-activated protein kinase 2
• UniProt Gene Name: Mapkapk2
• UniProt Synonym Gene Names: Rps6kc1; MAPK-activated protein kinase 2; MAPKAP kinase 2; MAPKAP-K2; MAPKAPK-2; MK-2; MK2

Uniprot Description

Stress-activated serine/threonine-protein kinase involved in cytokine production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X2-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, CEP131, ELAVL1, HNRNPA0, HSP27/HSPB1, KRT18, KRT20, LIMK1, LSP1, PABPC1, PARN, PDE4A, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Phosphorylates HSF1; leading to the interaction with HSP90 proteins and inhibiting HSF1 homotrimerization, DNA-binding and transactivation activities (). Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociation of HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impairment of their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins ELAVL1, HNRNPA0, PABPC1 and TTP/ZFP36, leading to regulation of the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcripts. Phosphorylates CEP131 in response to cellular stress following ultraviolet irradiation which promotes binding of CEP131 to 14-3-3 proteins and inhibits formation of novel centriolar satellites (). Also involved in late G2/M checkpoint following DNA damage through a process of post-transcriptional mRNA stabilization: following DNA damage, relocalizes from nucleus to cytoplasm and phosphorylates HNRNPA0 and PARN, leading to stabilization of GADD45A mRNA. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3.