Western Blot (WB)
(Western blot analysis of GAPDH in A431, Daudi, HepG2, HL60, Jurkat, Human kidney, Mouse lung, and Chicken liver lysate with GAPDH antibody 0.5 μg/mL.)
Rabbit GAPDH Polyclonal Antibody | anti-GAPDH antibody
GAPDH Antibody
Gene Names
GAPDH; G3PD; GAPD
Reactivity
Human, mouse, rat
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity chromatography purified via peptide column
Synonyms
GAPDH; Polyclonal Antibody; GAPDH Antibody; Glyceraldehyde-3-phosphate dehydrogenase; G3PDH; GAPD; (CT) an enzyme in the glycolytic pathway; anti-GAPDH antibody
Host
Rabbit
Reactivity
Human, mouse, rat
Clonality
Polyclonal
Purity/Purification
Affinity chromatography purified via peptide column
Form/Format
Liquid. PBS containing 0.02% sodium azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
335
Applicable Applications for anti-GAPDH antibody
ELISA, Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
GAPDH antibody can be used for the detection of GAPDH by Western Blot at 0.5 - 1 ug/mL.
Immunogen
16 amino acid peptide from near the carboxy terminus of human GAPDH.
Blocking Peptide
MBS152359 GAPDH Peptide
Positive Control 1
MBS151603 HeLa Lysate
Preparation and Storage
Store at 4 degree C for three months and -20 degree C. Stable for up to one year.
Western Blot (WB)
(Western blot analysis of GAPDH in A431, Daudi, HepG2, HL60, Jurkat, Human kidney, Mouse lung, and Chicken liver lysate with GAPDH antibody 0.5 μg/mL.)
Western Blot (WB)
(Western blot analysis of GAPDH in A431, Daudi, HepG2, HL60, Jurkat, Human kidney, Mouse lung, and Chicken liver lysate with GAPDH antibody 0.5 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemical staining of GAPDH in HeLa cells at a dilution of 10 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemical staining of GAPDH in HeLa cells at a dilution of 10 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of GAPDH in HeLa cells with GAPDH antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of GAPDH in HeLa cells with GAPDH antibody at 20 μg/mL.)
Related Product Information for anti-GAPDH antibody
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export. GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
References
* Sirover MA. New nuclear functions of the glycolytic protein, glyceraldehyde-3-phosphate dehydrogenase, in mammalian cells. J. Cell. Biochem. 2005; 95:45-52.
* Glyceraldehyde-3-phosphate dehydrogenase, apoptosis, and neurodegenerative diseases. Annu. Rev. Pharmacol. Toxicol. 2005; 45:269-90.
* Glyceraldehyde-3-phosphate dehydrogenase, apoptosis, and neurodegenerative diseases. Annu. Rev. Pharmacol. Toxicol. 2005; 45:269-90.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
36,053 Da
NCBI Official Full Name
Glyceraldehyde-3-phosphate dehydrogenase
NCBI Official Synonym Full Names
glyceraldehyde-3-phosphate dehydrogenase
NCBI Official Symbol
GAPDH
NCBI Official Synonym Symbols
G3PD; GAPD
NCBI Protein Information
glyceraldehyde-3-phosphate dehydrogenase; aging-associated gene 9 protein; peptidyl-cysteine S-nitrosylase GAPDH
UniProt Protein Name
Glyceraldehyde-3-phosphate dehydrogenase
UniProt Gene Name
GAPDH
UniProt Synonym Gene Names
GAPD; GAPDH
UniProt Entry Name
G3P_HUMAN
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Product Notes
The GAPDH gapdh (Catalog #AAA150873) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GAPDH Antibody reacts with Human, mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's GAPDH can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot (WB), Immunohistochemistry (IHC). GAPDH antibody can be used for the detection of GAPDH by Western Blot at 0.5 - 1 ug/mL. Researchers should empirically determine the suitability of the GAPDH gapdh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAPDH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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