Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (All lanes: Mouse anti-human ENO2 monoclonal antibody at 1ug/mlLane 1:Hela cell lysateLane 2:Recombinant Human Gamma-enolase(ENO2) at 10ugLane 3:U251 cell lysateSecondary Goat polyclonal to Mouse IgG at 1/3000 dilutionPredicted band size: 43,48 kDObserved band size: 48 kDAdditional bands at: 100kDa.)

Mouse Gamma-enolase Monoclonal Antibody | anti-ENO2/NSE antibody

Mouse anti Human Gamma-enolase(ENO2/NSE) monoclonal Antibody

Gene Names
ENO2; NSE; HEL-S-279
Reactivity
Human. Other species are not tested. Please decide the specificity by homology
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
>95%, Protein G Purified
Synonyms
Gamma-enolase; Monoclonal Antibody; Mouse anti Human Gamma-enolase(ENO2/NSE) monoclonal Antibody; 2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE; ENO2; anti-ENO2/NSE antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human. Other species are not tested. Please decide the specificity by homology
Clonality
Monoclonal
Isotype
IgG2a
Specificity
Specific for Human ENO2 denatured and native forms
Purity/Purification
>95%, Protein G Purified
Sequence Length
434
Applicable Applications for anti-ENO2/NSE antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Conjugate
Non-conjugated
Immunogen
Recombinant Human ENO2
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-56384 / sc-21737 / sc-21738 / sc-51880 / sc-71047 / sc-71046 / sc-376375
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(All lanes: Mouse anti-human ENO2 monoclonal antibody at 1ug/mlLane 1:Hela cell lysateLane 2:Recombinant Human Gamma-enolase(ENO2) at 10ugLane 3:U251 cell lysateSecondary Goat polyclonal to Mouse IgG at 1/3000 dilutionPredicted band size: 43,48 kDObserved band size: 48 kDAdditional bands at: 100kDa.)

Western Blot (WB) (All lanes: Mouse anti-human ENO2 monoclonal antibody at 1ug/mlLane 1:Hela cell lysateLane 2:Recombinant Human Gamma-enolase(ENO2) at 10ugLane 3:U251 cell lysateSecondary Goat polyclonal to Mouse IgG at 1/3000 dilutionPredicted band size: 43,48 kDObserved band size: 48 kDAdditional bands at: 100kDa.)

Immunohistochemistry (IHC)

(Immunohistochemical of paraffin-embedded human small intestine using MBS1499580 at dilution of 1:200)

Immunohistochemistry (IHC) (Immunohistochemical of paraffin-embedded human small intestine using MBS1499580 at dilution of 1:200)

Immunohistochemistry (IHC)

(Immunohistochemical of paraffin-embedded human pancreas using MBS1499580 at dilution of 1:200)

Immunohistochemistry (IHC) (Immunohistochemical of paraffin-embedded human pancreas using MBS1499580 at dilution of 1:200)
Related Product Information for anti-ENO2/NSE antibody
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
42,707 Da
NCBI Official Full Name
gamma-enolase
NCBI Official Synonym Full Names
enolase 2 (gamma, neuronal)
NCBI Official Symbol
ENO2
NCBI Official Synonym Symbols
NSE; HEL-S-279
NCBI Protein Information
gamma-enolase
UniProt Protein Name
Gamma-enolase
Protein Family
UniProt Gene Name
ENO2
UniProt Synonym Gene Names
NSE
UniProt Entry Name
ENOG_HUMAN

NCBI Description

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]

Uniprot Description

ENO2: an enzyme with 2-phospho-D-glycerate hydro-lyase activity. One of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 4.2.1.11; Lyase

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: cytosol; extracellular space; myelin sheath; perikaryon; phosphopyruvate hydratase complex; photoreceptor inner segment; plasma membrane

Molecular Function: magnesium ion binding; phosphopyruvate hydratase activity; protein binding

Biological Process: carbohydrate metabolic process; gluconeogenesis; glucose metabolic process

Research Articles on ENO2/NSE

Similar Products

Product Notes

The ENO2/NSE eno2 (Catalog #AAA1499580) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Human Gamma-enolase(ENO2/NSE) monoclonal Antibody reacts with Human. Other species are not tested. Please decide the specificity by homology and may cross-react with other species as described in the data sheet. AAA Biotech's Gamma-enolase can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the ENO2/NSE eno2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Gamma-enolase, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.