Rabbit anti-Human GALNT3 Polyclonal Antibody | anti-GALNT3 antibody

GALNT3, ID (GALNT3, Polypeptide N-acetylgalactosaminyltransferase 3, Polypeptide GalNAc transferase 3, Protein-UDP acetylgalactosaminyltransferase 3, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3)

Cat. Num. AAA648688

• Gene Names: GALNT3; HHS; HFTC; GalNAc-T3
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: GALNT3; Polyclonal Antibody; ID (GALNT3; Polypeptide N-acetylgalactosaminyltransferase 3; Polypeptide GalNAc transferase 3; Protein-UDP acetylgalactosaminyltransferase 3; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3); Anti -GALNT3; anti-GALNT3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-GALNT3 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: GALNT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 461~490 amino acids from the Center region of human GALNT3.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in MCF-7 cell line lysates (35ug/lane) using MBS648688. GALNT3 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human skin tissue reacted with MBS648688, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS648688 for immunohistochemistry.)

Testing Data

Related Product Information for anti-GALNT3 antibody

GALNT3 encodes UDP-GalNAc transferase 3, a member of the GalNAc-transferases family. This family transfers an N-acetyl galactosamine to the hydroxyl group of a serine or threonine residue in the first step of O-linked oligosaccharide biosynthesis. Individual GalNAc-transferases have distinct activities and initiation of O-glycosylation is regulated by a repertoire of GalNAc-transferases. The protein is highly homologous to other family members, however the enzymes have different substrate specificities.

Product Categories/Family for anti-GALNT3 antibody

Antibodies; Abs to Enzymes; Glycosyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 33604104
• NCBI GeneID: 2591
• UniProt Accession #: Q14435; Q53TG9; Q7Z476
• Molecular Weight: 72,610 Da
• NCBI Official Full Name: GALNT3 protein
• NCBI Official Synonym Full Names: UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 3 (GalNAc-T3)
• NCBI Official Symbol: GALNT3
• NCBI Official Synonym Symbols: HHS; HFTC; GalNAc-T3
• NCBI Protein Information: polypeptide N-acetylgalactosaminyltransferase 3; pp-GaNTase 3; GalNAc transferase 3; polypeptide GalNAc transferase 3; polypeptide GalNAc-transferase T3; protein-UDP acetylgalactosaminyltransferase 3; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3
• UniProt Protein Name: Polypeptide N-acetylgalactosaminyltransferase 3
• UniProt Gene Name: GALNT3
• UniProt Synonym Gene Names: GalNAc-T3
• UniProt Entry Name: GALT3_HUMAN

NCBI Description

This gene encodes UDP-GalNAc transferase 3, a member of the GalNAc-transferases family. This family transfers an N-acetyl galactosamine to the hydroxyl group of a serine or threonine residue in the first step of O-linked oligosaccharide biosynthesis. Individual GalNAc-transferases have distinct activities and initiation of O-glycosylation is regulated by a repertoire of GalNAc-transferases. The protein encoded by this gene is highly homologous to other family members, however the enzymes have different substrate specificities. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has activity toward HIV envelope glycoprotein gp120, EA2, Muc2 and Muc5. Probably glycosylates fibronectin in vivo. Glycosylates FGF23. Plays a central role in phosphate homeostasis. Ref.5 Ref.10

Catalytic activity: UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide. Ref.1

Cofactor: Manganese

By similarity.Calcium

By similarity.

Pathway: Protein modification; protein glycosylation.

Subcellular location: Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein. Note: Resides preferentially in the trans and medial parts of the Golgi stack. Ref.6

Tissue specificity: Expressed in organs that contain secretory epithelial glands. Highly expressed in pancreas, skin, kidney and testis. Weakly expressed in prostate, ovary, intestine and colon. Also expressed in placenta and lung and fetal lung and fetal kidney. Ref.1 Ref.7

Domain: There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding

By similarity.The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity

By similarity.

Involvement in disease: Tumoral calcinosis, hyperphosphatemic, familial (HFTC) [MIM:211900]: A severe metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Some patients manifest recurrent, transient, painful swellings of the long bones associated with the radiographic findings of periosteal reaction and cortical hyperostosis and absence of skin involvement.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.8 Ref.9

Miscellaneous: Overexpressed in many differentiated carcinomas, suggesting that it may serve as a marker of tumor differentiation.

Sequence similarities: Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.Contains 1 ricin B-type lectin domain.

Sequence caution: The sequence AAH56246.1 differs from that shown. Reason: Erroneous initiation.