Rabbit anti-Human Galectin-9 Polyclonal Antibody | anti-LGALS9 antibody

Galectin-9, ID (LGALS9, Gal-9, Ecalectin, Tumor Antigen HOM-HD-21)

Cat. Num. AAA6009120

• Gene Names: LGALS9; HUAT; LGALS9A
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Galectin-9; Polyclonal Antibody; ID (LGALS9; Gal-9; Ecalectin; Tumor Antigen HOM-HD-21); Anti -Galectin-9; anti-LGALS9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human LGALS9. Species sequence homology: mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-LGALS9 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.; Dilution: ELISA: 1:1000; Western Blot: 1:500-1:1000; Immunofluorescence: 1:100-1:500; Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
• Immunogen: Synthetic peptide corresponding to human LEG9.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(Western blot analysis of extracts from HepG2 cells, using LEG9 Antibody. The lane on the right is treated with the synthesized peptide.)

Immunofluorescence (IF)

(Immunofluorescence analysis of NIH-3T3 cells, using LEG9 Antibody. The picture on the right is treated with the synthesized peptide.)

Related Product Information for anti-LGALS9 antibody

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS9 is an S-type lectin. This galectin is strongly overexpressed in Hodgkin's disease tissue and it might participate in the interaction between the H&RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its consistently associated immunodeficiency. The protein has N- and C- terminal carbohydrate-binding domains connected by a link peptide. Two isoforms (long and short) exist.

Product Categories/Family for anti-LGALS9 antibody

Antibodies; Abs to Lectins

NCBI and Uniprot Product Information

• NCBI GI #: 102469694
• NCBI GeneID: 3965
• NCBI Accession #: NP_002299.2
• NCBI GenBank Nucleotide #: NM_002308.3
• UniProt Accession #: O00182; O14532; O75028; Q3B8N1; Q53FQ0; Q9NQ58; A7VJG6
• Molecular Weight: 39,518 Da
• NCBI Official Full Name: galectin-9 isoform short
• NCBI Official Synonym Full Names: lectin, galactoside-binding, soluble, 9
• NCBI Official Symbol: LGALS9
• NCBI Official Synonym Symbols: HUAT; LGALS9A
• NCBI Protein Information: galectin-9; gal-9; ecalectin; galectin 9; tumor antigen HOM-HD-21; urate transporter/channel protein
• UniProt Protein Name: Galectin-9
• Protein Family: Galectin
• UniProt Gene Name: LGALS9
• UniProt Synonym Gene Names: Gal-9
• UniProt Entry Name: LEG9_HUMAN

NCBI Description

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The protein encoded by this gene is an S-type lectin. It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H&RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its associated immunodeficiency. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

galectin-9: Binds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: cytoplasm

Molecular Function: signal transducer activity; galactose binding; carbohydrate binding

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; signal transduction