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Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human thyroid cancer using MBS769548 (Galectin 3 antibody) at dilution of 1:50)

Rabbit Galectin-3 Polyclonal Antibody

Galectin-3 Rabbit Polyclonal

Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunoprecipitation, Immunohistochemistry, Western Blot, Immunofluorescence
Purity
Purity: > = 95% as determined by SDS-PAGE
Purification: Immunogen Affinity Purified
Synonyms
Galectin-3; Polyclonal Antibody; Galectin-3 Rabbit Polyclonal; 35 kDa lectin; CBP 35; CBP35; Gal 3; GAL3; Galactose specific lectin 3; Galactoside binding protein; GALBP; Galectin 3; GALIG; IgE binding protein; L 31; Laminin binding protein; Lectin L 29; LGALS2; LGALS3; Mac 2 antigen; MAC2; anti-Galectin-3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Human, Mouse ,Rat; other species are not tested. Please decide the specificity by homology
Purity/Purification
Purity: > = 95% as determined by SDS-PAGE
Purification: Immunogen Affinity Purified
Form/Format
Liquid
Applicable Applications for anti-Galectin-3 antibody
ELISA (EIA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
Immunogen
lectin, galactoside-binding, soluble, 3
Buffer
PBS with 0.02% sodium azide and 50% glycerol pH 7.3
Preparation and Storage
-20 degree C for 24 months (Avoid repeated freeze/thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human thyroid cancer using MBS769548 (Galectin 3 antibody) at dilution of 1:50)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human thyroid cancer using MBS769548 (Galectin 3 antibody) at dilution of 1:50)

Immunofluorescence (IF)

(Immunofluorescent analysis of MCF-7 cells, using LGALS3 antibody MBS769548 at 1:50 dilution and Rhodamine-labeled goat anti-mouse IgG (red) .)

Immunofluorescence (IF) (Immunofluorescent analysis of MCF-7 cells, using LGALS3 antibody MBS769548 at 1:50 dilution and Rhodamine-labeled goat anti-mouse IgG (red) .)

Western Blot (WB)

(MCF-7 cells were subjected to SDS PAGE followed by western blot with MBS769548 (Galectin 3 Antibody) at dilution of 1:1500)

Western Blot (WB) (MCF-7 cells were subjected to SDS PAGE followed by western blot with MBS769548 (Galectin 3 Antibody) at dilution of 1:1500)
Related Product Information for anti-Galectin-3 antibody
Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for beta-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3 contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers. Galectin-3 is widely expressed in many normal tissues and a variety of tumors.

NCBI and Uniprot Product Information

UniProt Accession #
Molecular Weight
31 kDa
Protein Family

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Product Notes

The Galectin-3 (Catalog #AAA769548) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Galectin-3 Rabbit Polyclonal reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Galectin-3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blot (WB), Immunofluorescence (IF). WB: 1:500-1:5000 IP: 1:200-1:2000 IHC: 1:20-1:200 IF: 1:10-1:100. Researchers should empirically determine the suitability of the Galectin-3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Galectin-3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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