Rabbit anti-Human Galectin-1 Monoclonal Antibody | anti-LGALS1 antibody

Recombinant Anti-Galectin-1 Antibody, Rabbit Monoclonal

Cat. Num. AAA8102127

• Gene Names: LGALS1; GBP; GAL1
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A
• Synonyms: Galectin-1; Monoclonal Antibody; Recombinant Anti-Galectin-1 Antibody; Rabbit Monoclonal; Galectin-1/LGALS1 Antibody; Rabbit MAb; lectin; galactoside-binding; soluble; 1; Anti-GAL1 Antibody; Anti-Galectin-1 Antibody; Anti-GBP Antibody; anti-LGALS1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 001
• Specificity: Human Galectin-1/LGALS1
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-LGALS1 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IP: 1-4uL/mg of lysate
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Human Galectin-1/LGALS1 protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human Galectin-1/LGALS1 (rh Galectin-1/LGALS1).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-Galectin1 rabbit monoclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: K562 Whole Cell LysateLane C: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:14 kDaObserved band size:14 kDa)

Immunoprecipitation (IP)

(Galectin1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg K562 Whole Cell LysateLane C:0.5 mg A431 Whole Cell LysateLane D:0.5 mg HL-60 Whole Cell Lysate2 uL anti-Galectin1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Galectin1 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 15 kDaObserved band size: 15 kDa)

Related Product Information for anti-LGALS1 antibody

Galectin-1 (Gal-1, GAL1), is a member of the galectins, a family of animal lectins ranging from Caenorhabditis elegans to humans, which is defined by their affinity for beta-galactosides and by significant sequence similarity in the carbohydrate-binding site. It is a homodimer with a subunit molecular mass of 14.5 kDa, which contains six cysteine residues per subunit. The cysteine residues should be in a free state to maintain a molecular structure that is capable of showing lectin activity. This endogenous lectin widely expressed at sites of inflammation and tumor growth has been postulated as an attractive immunosuppressive agent to restore immune cell tolerance and homeostasis in autoimmune and inflammatory settings. On the other hand, galectin-1 contributes to different steps of tumor progression including cell adhesion, migration, and tumor-immune escape, suggesting that blockade of galectin-1 might result in therapeutic benefits in cancer. Several potential glycoprotein ligands for galectin-1 have been identified, including lysosome-associated membrane glycoproteins and fibronectin, laminin, as well as T-cell glycoproteins CD43 and CD45. Evidence points to Gal-1 and its ligands as one of the master regulators of such immune responses as T-cell homeostasis and survival, T-cell immune disorders, inflammation, and allergies as well as host-pathogen interactions.

References

Gaudet AD, et al. (2005) Expression and functions of galectin-1 in sensory and motoneurons. Curr Drug Targets. 6(4): 419-25.Kadoya T, et al. (2006) Structural and functional studies of galectin-1: a novel axonal regeneration-promoting activity for oxidized galectin-1. Curr Drug Targets. 6(4): 375-83.Camby I, et al. (2006) Galectin-1: a small protein with major functions. Glycobiology. 16(11): 137R-157R.Salatino M, et al. (2008) Galectin-1 as a potential therapeutic target in autoimmune disorders and cancer. Expert Opin Biol Ther. 8(1): 45-57.

NCBI and Uniprot Product Information

• NCBI GI #: 4504981
• NCBI GeneID: 3956
• NCBI Accession #: NP_002296.1
• NCBI GenBank Nucleotide #: NM_002305.3
• UniProt Accession #: P09382; Q9UDK5; B2R5E8
• Molecular Weight: 14,716 Da
• NCBI Official Full Name: galectin-1
• NCBI Official Synonym Full Names: lectin, galactoside-binding, soluble, 1
• NCBI Official Symbol: LGALS1
• NCBI Official Synonym Symbols: GBP; GAL1
• NCBI Protein Information: galectin-1; HBL; HPL; gal-1; HLBP14; galaptin; galectin 1; 14 kDa lectin; S-Lac lectin 1; lactose-binding lectin 1; 14 kDa laminin-binding protein; putative MAPK-activating protein PM12; beta-galactoside-binding lectin L-14-I; beta-galactoside-binding pro
• UniProt Protein Name: Galectin-1
• Protein Family: Galectin
• UniProt Gene Name: LGALS1
• UniProt Synonym Gene Names: Gal-1; HLBP14
• UniProt Entry Name: LEG1_HUMAN

NCBI Description

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation. [provided by RefSeq, Jul 2008]

Uniprot Description

Galectin-1: May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Homodimer. Binds LGALS3BP. Interacts with CD2, CD3, CD4, CD7, CD43 and CD45. Interacts with laminin (via poly-N- acetyllactosamine). Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion. Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.

Protein type: Cell adhesion

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; cell surface; cytoplasm; intracellular; nucleus

Molecular Function: protein binding; signal transducer activity; protein homodimerization activity; lactose binding; laminin binding; galactoside binding; glycoprotein binding

Biological Process: myoblast differentiation; response to drug; regulation of apoptosis; plasma cell differentiation; positive regulation of I-kappaB kinase/NF-kappaB cascade; apoptosis; T cell costimulation; positive regulation of erythrocyte aggregation; response to axon injury; signal transduction; multicellular organismal response to stress