Rabbit anti-Human, Mouse GAK Polyclonal Antibody | anti-GAK antibody

GAK (Cyclin-G-associated Kinase, FLJ16629, FLJ40395, MGC99654) (HRP)

Cat. Num. AAA6379190

• Gene Names: GAK; DNAJ26; DNAJC26
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GAK; Polyclonal Antibody; GAK (Cyclin-G-associated Kinase; FLJ16629; FLJ40395; MGC99654) (HRP); anti-GAK antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GAK. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 1775

• Applicable Applications for anti-GAK antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human GAK, aa1-416 (AAH08668.1).
• Immunogen Sequence: MSLLQSALDFLAGPGSLGGASGRDQSDFVGQTVELGELRLLLASPAPPLSVQSTPRGGPPAAADPFGPLLPSSGNNSQPCSNPDLFGEFLNSDSVTVPPSFPSAHSSPPPSCSADFLHLGDLPGEPSKMTASSSNPDLLGGWAAWTETAASAVAPTPATEGPLFSPGGQPAPCGSQASWTKSQNPDPFADLGDLSSGLQGSPAGFPPGGFIPKTATTPKGSSSWQTSRPPAQGASWPPQAKPPPKACTQPRPNYASNFSVIGAREERGVRAPSFAQKPKVSENDFEDLLSNQGFSSRSDKKGPKTIAEMRKQDLAKDTDPLKLKLLDWIEGKERNIRALLSTLHTVLWDGESRWTPVGMADLVAPEQVKKHYRRAVLAVHPDKAAGQPYEQHAKMIFMELNDAWSEFENQGSRPLF
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GAK rabbit polyclonal antibody. Western Blot analysis of GAK expression in mouse testis.)

Western Blot (WB)

(Western Blot analysis of GAK expression in transfected 293T cell line by GAK polyclonal antibody. Lane 1: GAK transfected lysate (44kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-GAK antibody

GAK, a member of the Ser/Thr protein kinase family, associates with cyclin G and CDK5. It appears to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1. GAK localizes to the perinuclear area and to the trans-Golgi network. It is also observed on the plasma membrane, probably at focals adhesions. Expression is ubiquitous, wiht highest levels in testis. The protein contains 1 J domain and 1 tensin domain.

Product Categories/Family for anti-GAK antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 14250461
• NCBI GeneID: 2580
• NCBI Official Full Name: Homo sapiens cyclin G associated kinase, mRNA
• NCBI Official Synonym Full Names: cyclin G associated kinase
• NCBI Official Symbol: GAK
• NCBI Official Synonym Symbols: DNAJ26; DNAJC26
• NCBI Protein Information: cyclin-G-associated kinase
• Protein Family: Cyclin-G-associated kinase

NCBI Description

In all eukaryotes, the cell cycle is governed by cyclin-dependent protein kinases (CDKs), whose activities are regulated by cyclins and CDK inhibitors in a diverse array of mechanisms that involve the control of phosphorylation and dephosphorylation of Ser, Thr or Tyr residues. Cyclins are molecules that possess a consensus domain called the 'cyclin box.' In mammalian cells, 9 cyclin species have been identified, and they are referred to as cyclins A through I. Cyclin G is a direct transcriptional target of the p53 tumor suppressor gene product and thus functions downstream of p53. GAK is an association partner of cyclin G and CDK5. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]