Rabbit anti-Human, Mouse GAK Polyclonal Antibody | anti-GAK antibody

GAK, NT (Cyclin-G-associated Kinase, FLJ16629, FLJ40395, MGC99654) (APC)

Cat. Num. AAA6477383

• Gene Names: GAK; DNAJ26; DNAJC26
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: GAK; Polyclonal Antibody; NT (Cyclin-G-associated Kinase; FLJ16629; FLJ40395; MGC99654) (APC); EC=2.7.11.1; Cyclin G Associated Kinase; anti-GAK antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GAK. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 587

• Applicable Applications for anti-GAK antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human GAK (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(Western blot analysis of anti-hGAK-G30 Pab in mouse heart tissue lysate. hGAK-G30(arrow) was detected using the purified Pab.)

Product Categories/Family for anti-GAK antibody

Antibodies; Protein Kinases

References

1. Strausberg, R.L., et al., Proc. Natl. Acad. Sci. USA 99(26):16899-16903 (2002). 2. Greener, T., et al., J. Biol. Chem. 275(2):1365-1370 (2000). 3. Kimura, S.H., et al., Genomics 44(2):179-187 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 39645325
• NCBI GeneID: 2580
• UniProt Accession #: Q6P490; O14976
• Molecular Weight: 62.076
• NCBI Official Full Name: GAK protein
• NCBI Official Synonym Full Names: cyclin G associated kinase
• NCBI Official Symbol: GAK
• NCBI Official Synonym Symbols: DNAJ26; DNAJC26
• NCBI Protein Information: cyclin-G-associated kinase
• UniProt Protein Name: GAK protein
• Protein Family: Cyclin-G-associated kinase
• UniProt Entry Name: Q6P490_HUMAN

NCBI Description

In all eukaryotes, the cell cycle is governed by cyclin-dependent protein kinases (CDKs), whose activities are regulated by cyclins and CDK inhibitors in a diverse array of mechanisms that involve the control of phosphorylation and dephosphorylation of Ser, Thr or Tyr residues. Cyclins are molecules that possess a consensus domain called the 'cyclin box.' In mammalian cells, 9 cyclin species have been identified, and they are referred to as cyclins A through I. Cyclin G is a direct transcriptional target of the p53 tumor suppressor gene product and thus functions downstream of p53. GAK is an association partner of cyclin G and CDK5. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]