Rabbit anti-Human GAK Polyclonal Antibody | anti-GAK antibody

GAK, CT (Cyclin-G-associated Kinase, FLJ16629, FLJ40395, MGC99654) (AP)

Cat. Num. AAA6477371

• Gene Names: GAK; DNAJ26; DNAJC26
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: GAK; Polyclonal Antibody; CT (Cyclin-G-associated Kinase; FLJ16629; FLJ40395; MGC99654) (AP); anti-GAK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GAK.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 587

• Applicable Applications for anti-GAK antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human GAK (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GAK (arrow) using GAK Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GAK gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(GAK Antibody (V1155) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the GAK antibody detected the GAK protein (arrow).)

Western Blot (WB)

(GAK Antibody (V1155) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the GAK antibody detected the GAK protein (arrow).)

Product Categories/Family for anti-GAK antibody

Antibodies; Protein Kinases

References

1. Strausberg, R.L., et al., Proc. Natl. Acad. Sci. USA 99(26):16899-16903 (2002). 2. Greener, T., et al., J. Biol. Chem. 275(2):1365-1370 (2000). 3. Kimura, S.H., et al., Genomics 44(2):179-187 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 39645325
• NCBI GeneID: 2580
• UniProt Accession #: Q6P490; O14976
• Molecular Weight: 143.19
• NCBI Official Full Name: GAK protein
• NCBI Official Synonym Full Names: cyclin G associated kinase
• NCBI Official Symbol: GAK
• NCBI Official Synonym Symbols: DNAJ26; DNAJC26
• NCBI Protein Information: cyclin-G-associated kinase
• UniProt Protein Name: GAK protein
• Protein Family: Cyclin-G-associated kinase
• UniProt Entry Name: Q6P490_HUMAN

NCBI Description

In all eukaryotes, the cell cycle is governed by cyclin-dependent protein kinases (CDKs), whose activities are regulated by cyclins and CDK inhibitors in a diverse array of mechanisms that involve the control of phosphorylation and dephosphorylation of Ser, Thr or Tyr residues. Cyclins are molecules that possess a consensus domain called the 'cyclin box.' In mammalian cells, 9 cyclin species have been identified, and they are referred to as cyclins A through I. Cyclin G is a direct transcriptional target of the p53 tumor suppressor gene product and thus functions downstream of p53. GAK is an association partner of cyclin G and CDK5. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]