Rabbit anti-Human, Mouse GAK Polyclonal Antibody | anti-GAK antibody

Rabbit Polyclonal (IgG) to Human GAK

Cat. Num. AAA248516

• Gene Names: GAK; DNAJ26; DNAJC26
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: GAK; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human GAK; Anti-GAK Antibody (aa101-150) IHC-plus; Auxilin-2; Cyclin-G-associated kinase; Cyclin G associated kinase; DNAJ26; DNAJC26; Human GAK; anti-GAK antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: GAK Antibody detects endogenous levels of total GAK protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1311

• Applicable Applications for anti-GAK antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:5000), IHC-P (20 ug/ml), WB (1:500 - 1:1000)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human GAK (aa101-150).
• Immunogen: GAK antibody was raised against synthetic peptide from human GAK (aa101-150).
• Antigen Modification: aa101-150
• Preparation and Storage: Store at -20 degree C for up to one year.

Immunohistochemistry (IHC)

(Anti-GAK antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.)

Western Blot (WB)

(Western blot of extracts from 293 cells, using GAK Antibody. The lane on the right is treated with the synthesized peptide.)

Related Product Information for anti-GAK antibody

In all eukaryotes, the cell cycle is governed by cyclin-dependent protein kinases (CDKs), whose activities are regulated by cyclins and CDK inhibitors in a diverse array of mechanisms that involve the control of phosphorylation and dephosphorylation of Ser, Thr or Tyr residues. Cyclins are molecules that possess a consensus domain called the 'cyclin box.' In mammalian cells, 9 cyclin species have been identified, and they are referred to as cyclins A through I.

Product Categories/Family for anti-GAK antibody

Family: Protein Kinase; Subfamily: NAK

NCBI and Uniprot Product Information

• NCBI GI #: 157384971
• NCBI GeneID: 2580
• NCBI Accession #: NP_005246.2
• NCBI GenBank Nucleotide #: NM_005255.2
• UniProt Accession #: O14976; Q5U4P5; Q9BVY6
• Molecular Weight: 134,464 Da
• NCBI Official Full Name: cyclin-G-associated kinase isoform 1
• NCBI Official Synonym Full Names: cyclin G associated kinase
• NCBI Official Symbol: GAK
• NCBI Official Synonym Symbols: DNAJ26; DNAJC26
• NCBI Protein Information: cyclin-G-associated kinase; auxilin-2
• UniProt Protein Name: Cyclin-G-associated kinase
• Protein Family: Cyclin-G-associated kinase
• UniProt Gene Name: GAK
• UniProt Entry Name: GAK_HUMAN

NCBI Description

In all eukaryotes, the cell cycle is governed by cyclin-dependent protein kinases (CDKs), whose activities are regulated by cyclins and CDK inhibitors in a diverse array of mechanisms that involve the control of phosphorylation and dephosphorylation of Ser, Thr or Tyr residues. Cyclins are molecules that possess a consensus domain called the 'cyclin box.' In mammalian cells, 9 cyclin species have been identified, and they are referred to as cyclins A through I. Cyclin G is a direct transcriptional target of the p53 tumor suppressor gene product and thus functions downstream of p53. GAK is an association partner of cyclin G and CDK5. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]

Uniprot Description

GAK: Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin- coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Protein type: Cell cycle regulation; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Other group; NAK family

Chromosomal Location of Human Ortholog: 4p16

Cellular Component: Golgi apparatus; focal adhesion; membrane; intracellular membrane-bound organelle; perinuclear region of cytoplasm

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding

Biological Process: endoplasmic reticulum organization and biogenesis; epidermal cell differentiation; cell cycle; forebrain morphogenesis; cell development; Golgi organization and biogenesis; protein amino acid phosphorylation