Rabbit anti-Human p90 RSK Polyclonal Antibody | anti-p90 RSK antibody

p90 RSK (Phospho-Thr573) Antibody

Cat. Num. AAA9402399

• Gene Names: RPS6KA1; RSK; HU-1; RSK1; p90Rsk; MAPKAPK1A
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: p90 RSK; Polyclonal Antibody; p90 RSK (Phospho-Thr573) Antibody; KS6A1; p90RSK1; RSK1; RPS6KA1; anti-p90 RSK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of P90RSK only when phosphorylated at threonine 573.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 744

• Applicable Applications for anti-p90 RSK antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500~1:1000; Immunohistochemistry: 1:50~1:100
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of threonine 573(L-M-T(p)-P-C) derived from Human p90 RSK.
• Modification: Phospho-Thr573
• Target Name: p90 RSK
• Preparation and Storage: Store at -20 degree C/1 year

Western Blot (WB)

(Western blot analysis of extracts from 293 cells treated with UV using p90 RSK (Phospho-Thr573) Antibody.The lane on the right is treated with the antigen-specific peptide.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p90 RSK (Phospho-Thr573) antibody (left)or the same antibody preincubated with blocking peptide (right).)

Related Product Information for anti-p90 RSK antibody

Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes.Eiichi Takahashi, J. Biol. Chem., Jul 1999; 274: 20206. Ye Zhang, J. Biol. Chem., May 2002; 277: 19042 - 19048. Ganesh R. Panta, Mol. Cell. Biol., Mar 2004; 24: 1823 - 1835.

Product Categories/Family for anti-p90 RSK antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 55743134
• NCBI GeneID: 6195
• NCBI Accession #: NP_001006666.1
• NCBI GenBank Nucleotide #: NM_001006665.1
• UniProt Accession #: Q15418; Q5SVM5; Q5SVM8; Q5SVM9; Q96C05; Q9BQK2; A6NGG4; A8K9K7; B2RDY8; B7Z5J0; E9PRI4
• Molecular Weight: 81,147 Da
• NCBI Official Full Name: ribosomal protein S6 kinase alpha-1 isoform b
• NCBI Official Synonym Full Names: ribosomal protein S6 kinase, 90kDa, polypeptide 1
• NCBI Official Symbol: RPS6KA1
• NCBI Official Synonym Symbols: RSK; HU-1; RSK1; p90Rsk; MAPKAPK1A
• NCBI Protein Information: ribosomal protein S6 kinase alpha-1
• UniProt Protein Name: Ribosomal protein S6 kinase alpha-1
• UniProt Gene Name: RPS6KA1
• UniProt Synonym Gene Names: MAPKAPK1A; RSK1; S6K-alpha-1; p90-RSK 1; p90RSK1; p90S6K; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; RSK-1
• UniProt Entry Name: KS6A1_HUMAN

NCBI Description

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

p90RSK: an AGC kinase of the RSK family. Phosphorylated and activated by Erk1 and -2 in response to many growth factors, polypeptide hormones and neurotransmitters. Several phosphorylation sites are important for its activation. Possesses two kinase domains connected by a regulator linker region. Phosphorylates a wide range of substrates including ribosomal protein S6. Prominently expressed in brain structures essential for cognitive function and learning.

Protein type: Translation; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; Kinase, protein; EC 2.7.11.1; AGC group; RSK family; RSK subfamily

Chromosomal Location of Human Ortholog: 1p

Cellular Component: nucleoplasm; cytoplasm; spindle; nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; caspase inhibitor activity; protein serine/threonine/tyrosine kinase activity; magnesium ion binding; ATP binding

Biological Process: regulation of transcription in response to stress; axon guidance; nerve growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; regulation of translation in response to stress; toll-like receptor 3 signaling pathway; negative regulation of caspase activity; signal transduction; cell cycle; protein amino acid phosphorylation; positive regulation of cell growth; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; synaptic transmission; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; positive regulation of cell differentiation; toll-like receptor 4 signaling pathway; negative regulation of apoptosis