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Immunohistochemistry (IHC) (Immunochemical staining of human GADD45G in human brain with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

Rabbit anti-Human GADD45G Polyclonal Antibody | anti-GADD45G antibody

Anti-GADD45G Antibody, Rabbit Polyclonal

Gene Names
GADD45G; CR6; DDIT2; GRP17; GADD45gamma
Reactivity
Human
Applications
ELISA, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
GADD45G; Polyclonal Antibody; Anti-GADD45G Antibody; Rabbit Polyclonal; GADD45G Antibody; Rabbit PAb; Antigen Affinity Purified; growth arrest and DNA-damage-inducible; gamma; Anti-CR6 Antibody; Anti-DDIT2 Antibody; Anti-GADD45gamma Antibody; Anti-GRP17 Antibody; anti-GADD45G antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human GADD45G
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-GADD45G antibody
ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
ELISA: 1:5000-1:10,000
IHC-P: 1:100-1:500
Immunogen
Recombinant Human GADD45G Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human GADD45G (rh GADD45G). GADD45G specific IgG was purified by Human GADD45G affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human GADD45G in human brain with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC) (Immunochemical staining of human GADD45G in human brain with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human GADD45G in human liver with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC) (Immunochemical staining of human GADD45G in human liver with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-GADD45G antibody
GADD45G, also known as CR6, is part of the nuclear proteins to interact with various proteins whose transcript levels are raised after stressful growth arrest conditions and treatment with DNA-damaging agents. GADD45G reacts to environmental stresses by mediating activation of the p38/JNK pathway which is mediated through their protein binding and activating MTK1/MEKK4 kinase, which is an upstream activator of both p38 and JNK MAPKs. GADD45G acts as a new-age tumor suppressor however is being frequently inactivated epigenetically in multiple tumors. GADD45G mRNA expression is down-regulated in hepatocellular carcinoma. GADD45G causes cell cycle arrest at G2/M transition when transfected into Hep-G2 cells. GADD45G induction by androgens involves new protein synthesis. Overexpression of GADD45G inhibits cell growth and causes morphological modifications in prostate cell lines thus GADD45G takes part in differentiation induction by androgens.
Product Categories/Family for anti-GADD45G antibody
References
Takekawa M. et al., 1998, Cell. 95 (4): 521-30. Suzuki M. et al., 1999, J Hum Genet. 44 (5): 300-3. Azam N. et al., 2001, J Biol Chem. 276 (4): 2766-74.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
17,121 Da
NCBI Official Full Name
growth arrest and DNA damage-inducible protein GADD45 gamma
NCBI Official Synonym Full Names
growth arrest and DNA-damage-inducible, gamma
NCBI Official Symbol
GADD45G
NCBI Official Synonym Symbols
CR6; DDIT2; GRP17; GADD45gamma
NCBI Protein Information
growth arrest and DNA damage-inducible protein GADD45 gamma; DDIT-2; GADD45-gamma; gadd-related protein, 17 kD; cytokine-responsive protein CR6; DNA damage-inducible transcript 2 protein
UniProt Protein Name
Growth arrest and DNA damage-inducible protein GADD45 gamma
UniProt Gene Name
GADD45G
UniProt Synonym Gene Names
CR6; DDIT2; DDIT-2
UniProt Entry Name
GA45G_HUMAN

NCBI Description

This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The GADD45G is highly expressed in placenta. [provided by RefSeq, Jul 2008]

Uniprot Description

GADD45G: Involved in the regulation of growth and apoptosis. Mediates activation of stress-responsive MTK1/MEKK4 MAPKKK. Belongs to the GADD45 family.

Protein type: Apoptosis; DNA repair, damage; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 9q22.1-q22.2

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding

Biological Process: activation of MAPKK activity; apoptosis; regulation of cell cycle; positive regulation of apoptosis; multicellular organismal development; activation of MAPKKK activity; positive regulation of JNK cascade; response to stress; negative regulation of protein kinase activity; cell differentiation

Research Articles on GADD45G

Similar Products

Product Notes

The GADD45G gadd45g (Catalog #AAA8107331) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GADD45G Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GADD45G can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P). ELISA: 1:5000-1:10,000 IHC-P: 1:100-1:500. Researchers should empirically determine the suitability of the GADD45G gadd45g for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GADD45G, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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