Rabbit CHOP Polyclonal Antibody | anti-CHOP antibody

CHOP (K121) Polyclonal Antibody

Cat. Num. AAA3000539

• Gene Names: DDIT3; CHOP; CEBPZ; CHOP10; CHOP-10; GADD153; AltDDIT3; C/EBPzeta
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: CHOP; Polyclonal Antibody; CHOP (K121) Polyclonal Antibody; DNA damage-inducible transcript 3 protein; DDIT-3; C/EBP zeta; C/EBP-homologous protein; C/EBP-homologous protein 10; CHOP-10; CCAAT/enhancer-binding protein homologous protein; Growth arrest and DNA damage-inducible protein GADD153; DDIT3; CHOP10; GADD153; anti-CHOP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CHOP (K121) polyclonal antibody detects endogenous levels of CHOP protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 169

• Applicable Applications for anti-CHOP antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 100-150 of Human CHOP.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of CHOP (K121) pAb at 1:500 dilutionLane1:A375 whole cell lysate (40ug) Lane2:HCT116 whole cell lysate (40ug) Lane3:The Kidney tissue lysate of Mouse (40ug) Lane4:PC12 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of CHOP (K121) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-CHOP antibody

GADD153 is a small nuclear protein that is capable of dimerizing with transcription factors C/EBP alpha and beta. Once dimerized, this complex inhibits the normal binding and function of C/EBP to classical binding sites. Inversely, the C/EBP GADD153 dimer gains binding activity to other non classical C/EBP stress related targets. Under normal cellular conditions this protein is not expressed in detectable levels, but is highly unregulated during times of cellular/ER stress. Examples of GADD153 inducing stress include: treatment with tunicamycin, nutrient starvation and reducing agents that interfere with the calcium flux across the ER membrane.

NCBI and Uniprot Product Information

• NCBI GI #: 304282234
• NCBI GeneID: 1649
• NCBI Accession #: NP_001181986.1
• NCBI GenBank Nucleotide #: NP_001181986.1
• UniProt Accession #: P35638; P0DPQ6
• Molecular Weight: ~ 19, 27kDa
• NCBI Official Full Name: DNA damage-inducible transcript 3 protein isoform 2
• NCBI Official Synonym Full Names: DNA damage inducible transcript 3
• NCBI Official Symbol: DDIT3
• NCBI Official Synonym Symbols: CHOP; CEBPZ; CHOP10; CHOP-10; GADD153; AltDDIT3; C/EBPzeta
• NCBI Protein Information: DNA damage-inducible transcript 3 protein
• UniProt Protein Name: DNA damage-inducible transcript 3 protein
• UniProt Gene Name: DDIT3
• UniProt Synonym Gene Names: CHOP; CHOP10; GADD153; DDIT-3; CHOP; CHOP-10
• UniProt Entry Name: DDIT3_HUMAN

NCBI Description

This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010]

Uniprot Description

CHOP: a transcriptional-regulatory protein of the bZIP family. Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA. May play an important role in melanoma progression. CK2-mediated phosphorylation inhibits its transcriptional activity. Up-regulates IL-6 transcription by trapping negative regulating NF-IL6 isoform.

Protein type: Autophagy; Transcription factor; DNA-binding; Oncoprotein

Chromosomal Location of Human Ortholog: 12q13.1-q13.2

Cellular Component: nucleoplasm; late endosome; nucleus; cytosol

Molecular Function: leucine zipper domain binding; protein binding; DNA binding; protein heterodimerization activity; transcription factor binding; transcription factor activity; transcription corepressor activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-8 production; regulation of transcription, DNA-dependent; positive regulation of neuron apoptosis; cell cycle arrest; ER overload response; regulation of transcription in response to stress; negative regulation of protein kinase B signaling cascade; Wnt receptor signaling pathway; unfolded protein response; negative regulation of transcription factor activity; blood vessel maturation; mRNA transcription from RNA polymerase II promoter; response to unfolded protein; response to starvation; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; cell redox homeostasis; release of sequestered calcium ion into cytosol; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; inhibition of CREB transcription factor; negative regulation of myoblast differentiation; response to DNA damage stimulus

Disease: Myxoid Liposarcoma