Rabbit GAD67 Polyclonal Antibody | anti-GAD67 antibody

GAD67 Polyclonal Antibody

Cat. Num. AAA3012737

• Gene Names: GAD1; GAD; SCP; CPSQ1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation
• Purity: ProA affinity purified
• Synonyms: GAD67; Polyclonal Antibody; GAD67 Polyclonal Antibody; GAD-67; 67 kDa glutamic acid decarboxylase; CPSQ1; DCE1; EC 4.1.1.15; FLJ45882; GAD 67; GAD; GAD1; Glutamate decarboxylase 1 (brain; 67kDa); Glutamate decarboxylase 1; Glutamate decarboxylase 1 brain 67kD; Glutamate decarboxylase 1 brain 67kDa; Glutamate decarboxylase 67 kDa isoform; Glutamate decarboxylase; brain; 67-KD; OTTHUMP00000041055; SCP; anti-GAD67 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: GAD67 polyclonal antibody detects endogenous levels of GAD67 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 594

• Applicable Applications for anti-GAD67 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1,000; IP: 1:50-1:100
• Immunogen: recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of GAD67 on Hela cells lysates using anti-GAD67 antibody at 1/500 dilution.)

Related Product Information for anti-GAD67 antibody

There are two forms of glutamic acid decarboxylases (GADs) that are found in the brain: GAD-65 (also known as GAD2) and GAD-67 (also known as GAD1, GAD or SCP). GAD-65 and GAD-67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate limiting step in the production of GABA (g-aminobutyric acid) from L-glutamic acid. Although both GADs are found in the brain, GAD-65 localizes to synaptic vesicle membranes in nerve terminals, while GAD-67 is distributed throughout the cell. GAD-67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD-65 will transiently activate to assist in GABA production. The loss of GAD-65 is detrimental and can impair GABA neurotransmission, however the loss of GAD-67 is lethal. Due to alternative splicing, two isoforms exist for GAD-67, the predominant GAD-67 form and the minor GAD-25 form. GAD-25 is not expressed in brain but can be found in a variety of endocrine tissues.

NCBI and Uniprot Product Information

• NCBI GI #: 58331246
• NCBI GeneID: 2571
• NCBI Accession #: NP_000808.2
• NCBI GenBank Nucleotide #: NP_000808.2
• UniProt Accession #: Q99259; P48318; P18088
• Molecular Weight: 67kDa
• NCBI Official Full Name: glutamate decarboxylase 1 isoform GAD67
• NCBI Official Synonym Full Names: glutamate decarboxylase 1
• NCBI Official Symbol: GAD1
• NCBI Official Synonym Symbols: GAD; SCP; CPSQ1
• NCBI Protein Information: glutamate decarboxylase 1
• UniProt Protein Name: Glutamate decarboxylase 1
• UniProt Gene Name: GAD1
• UniProt Synonym Gene Names: GAD; GAD67; GAD-67
• UniProt Entry Name: DCE1_HUMAN

NCBI Description

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008]

Uniprot Description

GAD67: Catalyzes the production of GABA. Homodimer. Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. Belongs to the group II decarboxylase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Other Amino Acids Metabolism - taurine and hypotaurine; Other Amino Acids Metabolism - beta-alanine; Carbohydrate Metabolism - butanoate; Lyase; EC 4.1.1.15; Mitochondrial

Chromosomal Location of Human Ortholog: 2q31

Cellular Component: plasma membrane; intracellular; vesicle membrane

Molecular Function: protein binding; glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; protein N-terminus binding; pyridoxal phosphate binding

Biological Process: response to drug; glutamate catabolic process; synaptic transmission; glutamate decarboxylation to succinate; neurotransmitter secretion; gamma-aminobutyric acid biosynthetic process; protein-pyridoxal-5-phosphate linkage; neurotransmitter biosynthetic process

Disease: Cerebral Palsy, Spastic Quadriplegic, 1