Goat anti-Human GAD65 Polyclonal Antibody | anti-GAD2 antibody

GAD65, ID (Glutamate Decarboxylase 2, 65kD Glutamic Acid Decarboxylase, GAD-65, Glutamate Decarboxylase 65kD Isoform, GAD2)

Cat. Num. AAA6001753

• Gene Names: GAD2; GAD65
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: GAD65; Polyclonal Antibody; ID (Glutamate Decarboxylase 2; 65kD Glutamic Acid Decarboxylase; GAD-65; Glutamate Decarboxylase 65kD Isoform; GAD2); Anti -GAD65; anti-GAD2 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human GAD65. Species sequence homology: Chimpanzee, canine, mouse, rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-GAD2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: ELISA: 1:32,000; Western Blot: 1:32,000 & 0.1-0.3ug/ml; Immunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml
• Immunogen: Synthetic peptide corresponding to C-TLEDNEERMSRLSK from an internal region of human GAD2.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Brain, cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry (IHC)

(Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))

Testing Data

(Antibody (0.1ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Product Categories/Family for anti-GAD2 antibody

Antibodies; Abs to Enzymes; Decarboxylase

NCBI and Uniprot Product Information

• NCBI GI #: 197276620
• NCBI GeneID: 2572
• NCBI Accession #: NP_001127838.1
• NCBI GenBank Nucleotide #: NM_001134366.1
• UniProt Accession #: Q05329; Q9UD87
• Molecular Weight: 65,411 Da
• NCBI Official Full Name: glutamate decarboxylase 2
• NCBI Official Synonym Full Names: glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
• NCBI Official Symbol: GAD2
• NCBI Official Synonym Symbols: GAD65
• NCBI Protein Information: glutamate decarboxylase 2; GAD-65; 65 kDa glutamic acid decarboxylase; Glutamate decarboxylase-2 (pancreas); glutamate decarboxylase 65 kDa isoform
• UniProt Protein Name: Glutamate decarboxylase 2
• Protein Family: Glutamate decarboxylase
• UniProt Gene Name: GAD2
• UniProt Synonym Gene Names: GAD65; GAD-65
• UniProt Entry Name: DCE2_HUMAN

NCBI Description

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]

Uniprot Description

GAD2: Catalyzes the production of GABA. Homodimer. Belongs to the group II decarboxylase family.

Protein type: Lyase; Carbohydrate Metabolism - butanoate; Amino Acid Metabolism - alanine, aspartate and glutamate; Other Amino Acids Metabolism - taurine and hypotaurine; Other Amino Acids Metabolism - beta-alanine; EC 4.1.1.15

Chromosomal Location of Human Ortholog: 10p11.23

Cellular Component: presynaptic membrane; Golgi membrane; synaptic vesicle membrane; perinuclear region of cytoplasm; axon; plasma membrane; cell junction; cytosol

Molecular Function: protein binding; glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; pyridoxal phosphate binding

Biological Process: response to drug; synaptic transmission; glutamate decarboxylation to succinate; neurotransmitter secretion; neurotransmitter biosynthetic process