Human GAD2 (Glutamate Decarboxylase 2) CLIA Kit | GAD2 clia kit

Human GAD2 (Glutamate Decarboxylase 2) CLIA Kit

Cat. Num. AAA2533059

• Gene Names: GAD2; GAD65
• Reactivity: Human
• Synonyms: GAD2 (Glutamate Decarboxylase 2); Human GAD2 (Glutamate Decarboxylase 2) CLIA Kit; GAD2 clia kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant GAD2. No significant cross-reactivity or interference between GAD2 and analogues was observed.
• Sequence Length: 585

• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GAD2 clia kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of GAD2 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to GAD2. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GAD2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GAD2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of GAD2. You can calculate the concentration of GAD2 in the samples by comparing the RLU value of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 197276620
• NCBI GeneID: 2572
• NCBI Accession #: NP_001127838.1
• NCBI GenBank Nucleotide #: NM_001134366.1
• Molecular Weight: 65,411 Da
• NCBI Official Full Name: glutamate decarboxylase 2
• NCBI Official Synonym Full Names: glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
• NCBI Official Symbol: GAD2
• NCBI Official Synonym Symbols: GAD65
• NCBI Protein Information: glutamate decarboxylase 2
• UniProt Protein Name: Glutamate decarboxylase 2
• Protein Family: Glutamate decarboxylase
• UniProt Gene Name: GAD2
• UniProt Synonym Gene Names: GAD65; GAD-65
• UniProt Entry Name: DCE2_HUMAN

NCBI Description

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]

Uniprot Description

GAD2: Catalyzes the production of GABA. Homodimer. Belongs to the group II decarboxylase family.

Protein type: Other Amino Acids Metabolism - beta-alanine; Carbohydrate Metabolism - butanoate; Lyase; Other Amino Acids Metabolism - taurine and hypotaurine; Amino Acid Metabolism - alanine, aspartate and glutamate; EC 4.1.1.15

Chromosomal Location of Human Ortholog: 10p11.23

Cellular Component: Golgi membrane; presynaptic membrane; synaptic vesicle membrane; perinuclear region of cytoplasm; axon; plasma membrane; cell junction; cytosol

Molecular Function: protein binding; glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; pyridoxal phosphate binding

Biological Process: response to drug; synaptic transmission; glutamate decarboxylation to succinate; neurotransmitter secretion; neurotransmitter biosynthetic process