Rabbit anti-Human GAD1 Polyclonal Antibody | anti-GAD1 antibody

GAD1, CT (GAD1, GAD, GAD67, Glutamate decarboxylase 1, 67kD glutamic acid decarboxylase, Glutamate decarboxylase 67kD isoform) (FITC)

Cat. Num. AAA6300942

• Gene Names: GAD1; GAD; SCP; CPSQ1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GAD1; Polyclonal Antibody; CT (GAD1; GAD; GAD67; Glutamate decarboxylase 1; 67kD glutamic acid decarboxylase; Glutamate decarboxylase 67kD isoform) (FITC); Glutamate decarboxylase 67kD isoform; anti-GAD1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-GAD1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: GAD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 521-551 amino acids from the C-terminal region of human GAD1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GAD1 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the GAD1 antibody detected the GAD1 protein (arrow).)

Related Product Information for anti-GAD1 antibody

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.

Product Categories/Family for anti-GAD1 antibody

Antibodies; Enzymes; Decarboxylase

References

Lanoue, A.C., et al. Exp. Neurol. 226(1):207-217(2010). Jia, P., et al. Schizophr. Res. 122 (1-3), 38-42 (2010): Terranova, C., et al. Alcohol 44(5):407-413(2010). Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010). Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 58331246
• NCBI GeneID: 2571
• NCBI Accession #: NP_000808
• NCBI GenBank Nucleotide #: NM_000817.2
• UniProt Accession #: Q99259; Q49AK1; Q53TQ7; Q9BU91; Q9UHH4
• Molecular Weight: 69.3 kDa (617aa)
• NCBI Official Full Name: glutamate decarboxylase 1 isoform GAD67
• NCBI Official Synonym Full Names: glutamate decarboxylase 1
• NCBI Official Symbol: GAD1
• NCBI Official Synonym Symbols: GAD; SCP; CPSQ1
• NCBI Protein Information: glutamate decarboxylase 1
• UniProt Protein Name: Glutamate decarboxylase 1
• Protein Family: Glutamate decarboxylase
• UniProt Gene Name: GAD1
• UniProt Synonym Gene Names: GAD; GAD67; GAD-67
• UniProt Entry Name: DCE1_HUMAN

NCBI Description

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008]

Uniprot Description

GAD67: Catalyzes the production of GABA. Homodimer. Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. Belongs to the group II decarboxylase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Other Amino Acids Metabolism - taurine and hypotaurine; Other Amino Acids Metabolism - beta-alanine; Carbohydrate Metabolism - butanoate; Lyase; EC 4.1.1.15; Mitochondrial

Chromosomal Location of Human Ortholog: 2q31

Cellular Component: plasma membrane; intracellular; vesicle membrane

Molecular Function: protein binding; glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; protein N-terminus binding; pyridoxal phosphate binding

Biological Process: response to drug; glutamate catabolic process; synaptic transmission; glutamate decarboxylation to succinate; neurotransmitter secretion; gamma-aminobutyric acid biosynthetic process; protein-pyridoxal-5-phosphate linkage; neurotransmitter biosynthetic process

Disease: Cerebral Palsy, Spastic Quadriplegic, 1