Rabbit SRF / Serum Response Factor Polyclonal Antibody | anti-SRF antibody

Rabbit Polyclonal (IgG) to Human SRF / Serum Response Factor

Cat. Num. AAA243385

• Gene Names: SRF; MCM1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: SRF / Serum Response Factor; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human SRF / Serum Response Factor; Anti-SRF / Serum Response Factor Antibody (aa71-120) IHC-plus; SRF; Serum response factor; MCM1; Human SRF; Serum Response Factor; anti-SRF antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SRF (Ab-99) Antibody detects endogenous levels of total SRF protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 508

• Applicable Applications for anti-SRF antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:5000), IHC-P (5 ug/ml), WB (1:500 - 1:1000)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human SRF (aa71-120).
• Immunogen: SRF / Serum Response Factor antibody was raised against synthetic peptide from human SRF (aa71-120).
• Antigen Modification: aa71-120
• Preparation and Storage: Store at -20 degree C for up to one year.

Immunohistochemistry (IHC)

(Anti-SRF antibody IHC of human prostate, fibromuscular stroma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.)

Western Blot (WB)

(Western blot of extracts from NIH-3T3 cells, treated with PMA 125 ng/ml 30', using SRF (Ab-99) Antibody. The lane on the right is treated with the synthesized peptide.)

Product Categories/Family for anti-SRF antibody

Family: Transcription factor

NCBI and Uniprot Product Information

• NCBI GI #: 4507205
• NCBI GeneID: 6722
• NCBI Accession #: NP_003122.1
• NCBI GenBank Nucleotide #: NM_003131.3
• UniProt Accession #: P11831; Q5T648
• Molecular Weight: 51,593 Da
• NCBI Official Full Name: serum response factor isoform 1
• NCBI Official Synonym Full Names: serum response factor (c-fos serum response element-binding transcription factor)
• NCBI Official Symbol: SRF
• NCBI Official Synonym Symbols: MCM1
• NCBI Protein Information: serum response factor
• UniProt Protein Name: Serum response factor
• Protein Family: Serum response factor
• UniProt Gene Name: SRF
• UniProt Synonym Gene Names: SRF
• UniProt Entry Name: SRF_HUMAN

NCBI Description

This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]

Uniprot Description

SRF: a transcription factor of the MADS domain family that binds to the serum response element (SRE). Regulates the transcription of immediate early genes including c-fos. Binds DNA as a multimer, probably a dimer.

Protein type: Motility/polarity/chemotaxis; Transcription factor

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: cytoplasm; nuclear chromatin; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein homodimerization activity; chromatin DNA binding; transcription factor binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; developmental growth; erythrocyte development; positive regulation of transcription by glucose; heart development; positive regulation of axon extension; cell-matrix adhesion; response to toxin; neuron migration; response to hormone stimulus; cardiac myofibril assembly; muscle maintenance; negative regulation of cell proliferation; tangential migration from the subventricular zone to the olfactory bulb; regulation of smooth muscle cell differentiation; morphogenesis of an epithelial sheet; heart looping; positive regulation of smooth muscle contraction; negative regulation of cell migration; associative learning; neurite development; positive thymic T cell selection; positive regulation of filopodium formation; regulation of cell adhesion; platelet activation; skin morphogenesis; hippocampus development; sarcomere organization; angiogenesis involved in wound healing; mRNA transcription from RNA polymerase II promoter; trophectodermal cell differentiation; regulation of water loss via skin; patterning of blood vessels; contractile actin filament bundle formation; cell migration during sprouting angiogenesis; mesoderm formation; long-term memory; response to cytokine stimulus; stress fiber formation; response to hypoxia; neuron development; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; platelet formation; positive regulation of cell differentiation