Rabbit anti-Human, Mouse GABRG3 Polyclonal Antibody | anti-GABRG3 antibody

GABRG3, NT (GABRG3, Gamma-aminobutyric acid receptor subunit gamma-3, GABA(A) receptor subunit gamma-3) (Azide free) (HRP)

Cat. Num. AAA6300910

• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GABRG3; Polyclonal Antibody; NT (GABRG3; Gamma-aminobutyric acid receptor subunit gamma-3; GABA(A) receptor subunit gamma-3) (Azide free) (HRP); GABA(A) receptor subunit gamma-3; anti-GABRG3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-GABRG3 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: GABRG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8~37 amino acids from the N-terminal region of human GABRG3.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GABRG3 Antibody (N-term) in mouse brain tissue lysates (35ug/lane). GABRG3 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(GABRG3 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GABRG3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(GABRG3 Antibody (N-term) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-GABRG3 antibody

GABRG3 is a family of proteins involved in the GABAergic neurotransmission of the mammalian central nervous system. GABRG3 is a member of the GABA-A receptor gene family of heteromeric pentameric ligand-gated ion channels through which GABA, the major inhibitory neurotransmitter in the mammalian brain, acts. GABA-A receptors are the site of action of a number of important pharmacologic agents including barbiturates, benzodiazepines, and ethanol (summary by Whiting et al., 1999 [PubMed 10414349]). For additional general information about the GABA-A receptor gene family, see GABRA1 (MIM 137160).

Product Categories/Family for anti-GABRG3 antibody

Antibodies; Receptors

References

Guilmatre, A., et al. Arch. Gen. Psychiatry 66(9):947-956(2009). Chakrabarti, B., et al. Autism Res 2(3):157-177(2009). Tabakoff, B., et al. BMC Biol. 7, 70 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 110347416
• NCBI GeneID: 2567
• NCBI Accession #: NP_150092.2
• NCBI GenBank Nucleotide #: NM_033223.4
• UniProt Accession #: Q99928; Q9HD46; Q9NYT2; G3V594
• Molecular Weight: 54,289 Da
• NCBI Official Full Name: gamma-aminobutyric acid receptor subunit gamma-3 isoform 1
• NCBI Official Synonym Full Names: gamma-aminobutyric acid (GABA) A receptor, gamma 3
• NCBI Official Symbol: GABRG3
• NCBI Protein Information: gamma-aminobutyric acid receptor subunit gamma-3; GABA(G) receptor, gamma 3; GABA(A) receptor subunit gamma-3
• UniProt Protein Name: Gamma-aminobutyric acid receptor subunit gamma-3
• Protein Family: Gamma-aminobutyric acid receptor
• UniProt Gene Name: GABRG3
• UniProt Entry Name: GBRG3_HUMAN

NCBI Description

This gene encodes a gamma-aminobutyric acid (GABA) receptor. GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. GABA-A receptors are pentameric, consisting of proteins from several subunit classes: alpha, beta, gamma, delta and rho. The protein encoded by this gene is a gamma subunit, which contains the benzodiazepine binding site. Two transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Aug 2012]

Uniprot Description

GABRG3: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRG3 sub-subfamily.

Protein type: Transporter, ion channel; Membrane protein, integral; Channel, ligand-gated; Transporter; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 15q12

Cellular Component: postsynaptic membrane; plasma membrane; cell junction

Molecular Function: chloride channel activity; GABA-A receptor activity; extracellular ligand-gated ion channel activity

Biological Process: response to drug; synaptic transmission; transmembrane transport; gamma-aminobutyric acid signaling pathway