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Western Blot (WB) (Western blot analysis of DIPA Antibody (Center) in HepG2 cell line lysates (35ug/lane). DIPA (arrow) was detected using the purified Pab.)

Rabbit DIPA Polyclonal Antibody | anti-CCDC85B antibody

DIPA Antibody (Center)

Gene Names
CCDC85B; DIPA
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
DIPA; Polyclonal Antibody; DIPA Antibody (Center); Coiled-coil domain-containing protein 85B; Hepatitis delta antigen-interacting protein A; Delta-interacting protein A; CCDC85B; anti-CCDC85B antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This DIPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 69-96 amino acids from the Central region of human DIPA.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
69-96
Sequence Length
202
Applicable Applications for anti-CCDC85B antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of DIPA Antibody (Center) in HepG2 cell line lysates (35ug/lane). DIPA (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of DIPA Antibody (Center) in HepG2 cell line lysates (35ug/lane). DIPA (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human skeletal muscle reacted with DIPA Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human skeletal muscle reacted with DIPA Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(DIPA Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (DIPA Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-CCDC85B antibody
DIPA functions as a transcriptional repressor. It may inhibit the activity of CTNNB1 in a TP53-dependent manner and thus regulate cell growth. It may function in adipocyte differentiation, negatively regulating mitotic clonal expansion.
Product Categories/Family for anti-CCDC85B antibody
References
Bezy,O., et.al., J. Biol. Chem. 280 (12), 11432-11438 (2005)
Du,X.,et.al., Exp. Mol. Pathol. 81 (3), 184-190 (2006)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22091
NCBI Official Full Name
coiled-coil domain-containing protein 85B
NCBI Official Synonym Full Names
coiled-coil domain containing 85B
NCBI Official Symbol
CCDC85B
NCBI Official Synonym Symbols
DIPA
NCBI Protein Information
coiled-coil domain-containing protein 85B
UniProt Protein Name
Coiled-coil domain-containing protein 85B
UniProt Gene Name
CCDC85B
UniProt Synonym Gene Names
DIPA; Delta-interacting protein A
UniProt Entry Name
CC85B_HUMAN

NCBI Description

Hepatitis delta virus (HDV) is a pathogenic human virus whose RNA genome and replication cycle resemble those of plant viroids. Delta-interacting protein A (DIPA), a cellular gene product, has been found to have homology to hepatitis delta virus antigen (HDAg). DIPA interacts with the viral antigen, HDAg, and can affect HDV replication in vitro. [provided by RefSeq, Jul 2008]

Uniprot Description

CCDC85B: Functions as a transcriptional repressor. May inhibit the activity of CTNNB1 in a TP53-dependent manner and thus regulate cell growth. May function in adipocyte differentiation, negatively regulating mitotic clonal expansion. Belongs to the CCDC85 family.

Protein type: Transcription regulation

Chromosomal Location of Human Ortholog: 11q12.1

Cellular Component: centrosome; cytoplasm; nucleus

Molecular Function: protein binding

Biological Process: transcription, DNA-dependent; negative regulation of cell growth; negative regulation of fat cell differentiation; negative regulation of transcription, DNA-dependent; cell differentiation

Research Articles on CCDC85B

Similar Products

Product Notes

The CCDC85B ccdc85b (Catalog #AAA9210923) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 69-96. The DIPA Antibody (Center) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's DIPA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the CCDC85B ccdc85b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DIPA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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