Rabbit anti-Human GABPA Polyclonal Antibody | anti-GABPA antibody

GABPA, ID (GABPA, E4TF1A, GA-binding protein alpha chain, Nuclear respiratory factor 2 subunit alpha, Transcription factor E4TF1-60) (Azide free) (HRP)

Cat. Num. AAA6300800

• Gene Names: GABPA; NFT2; NRF2; NRF2A; E4TF1A; E4TF1-60; RCH04A07
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GABPA; Polyclonal Antibody; ID (GABPA; E4TF1A; GA-binding protein alpha chain; Nuclear respiratory factor 2 subunit alpha; Transcription factor E4TF1-60) (Azide free) (HRP); Transcription factor E4TF1-60; anti-GABPA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 454

• Applicable Applications for anti-GABPA antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: GABPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 282-311 amino acids from the Central region of human GABPA.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GABPA Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the GABPA antibody detected the GABPA protein (arrow).)

Related Product Information for anti-GABPA antibody

This gene encodes one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype. Two transcript variants encoding the same protein have been found for this gene.

Product Categories/Family for anti-GABPA antibody

Antibodies; Transcription Factors

References

Henderson, A.J., et al. Thorax 65(10):897-902(2010). Eynon, N., et al. Physiol. Genomics 41(1):78-81(2010). Levy, S., et al. IUBMB Life 62(3):237-246(2010). Kim, Y.R., et al. J. Pathol. 220(4):446-451(2010). Bruni, F., et al. J. Biol. Chem. 285(6):3939-3948(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 34452721
• NCBI GeneID: 2551
• NCBI Accession #: NP_002031.2
• NCBI GenBank Nucleotide #: NP_002031.2
• UniProt Accession #: Q06546
• NCBI Official Full Name: GA-binding protein alpha chain
• NCBI Official Synonym Full Names: GA binding protein transcription factor subunit alpha
• NCBI Official Symbol: GABPA
• NCBI Official Synonym Symbols: NFT2; NRF2; NRF2A; E4TF1A; E4TF1-60; RCH04A07
• NCBI Protein Information: GA-binding protein alpha chain
• UniProt Protein Name: GA-binding protein alpha chain
• Protein Family: GA-binding protein
• UniProt Gene Name: GABPA
• UniProt Synonym Gene Names: E4TF1A; GABP subunit alpha
• UniProt Entry Name: GABPA_HUMAN

NCBI Description

This gene encodes one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Oct 2010]

Uniprot Description

GABPA: Transcription factor capable of interacting with purine rich repeats (GA repeats). Necessary for the expression of the Adenovirus E4 gene. Belongs to the ETS family.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 21q21.3

Cellular Component: nucleoplasm; nuclear chromatin; nucleus

Molecular Function: protein binding; DNA binding; protein heterodimerization activity; transcription coactivator activity; chromatin binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; mitochondrion organization and biogenesis; in utero embryonic development; organelle organization and biogenesis; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; cell differentiation; negative regulation of megakaryocyte differentiation