Rabbit anti-Human, Rat GABARAPL1 Polyclonal Antibody | anti-GABARAPL1 antibody

Rabbit Anti-Human GABARAPL1 Polyclonal

Cat. Num. AAA807688

• Gene Names: GABARAPL1; ATG8; GEC1; APG8L; ATG8B; ATG8L; APG8-LIKE
• Reactivity: Human, Rat
• Applications: Western Blot
• Purity: Peptide Affinity Purified
• Synonyms: GABARAPL1; Polyclonal Antibody; Rabbit Anti-Human GABARAPL1 Polyclonal; APG8 like Antibdoy; ATG8 Antibody; GABA(A) Receptor Associated Protein Like 1 Antibody; Glandular epithelial cell protein 1 Antibody; GABARAPL1 Antibody: RPE ; GABARAPL1 Antibody; anti-GABARAPL1 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Purity/Purification: Peptide Affinity Purified
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 117

• Applicable Applications for anti-GABARAPL1 antibody: Western Blot (WB)
• Application Notes: Western Blot: 1:1000
• Immunogen: Synthetic peptide from the C-terminal of human GABARAPL1
• Immunogen Species: Human
• COA: A 1:1000 dilution was sufficient for detection of GABARAPL1 on rat liver lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
• Storage Buffer: PBS, 50% glycerol, 0.09% sodium azide
• Conjugate: RPE
• Cellular Localization: Cytoplasm, Cytoskeleton, Endoplasmic Reticulum, Golgi Apparatus, Cytoplasmic Vesicle, Autophagosome
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~14kDa GABARAPL1 protein using Rabbit Anti-GABARAPL1 Polyclonal Antibody (SPC-623). Lane 1: MW Ladder. Lane 2: Human HeLa (20 ug). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-GABARAPL1 Polyclonal Antibody (SPC-623) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~14kDa.)

Related Product Information for anti-GABARAPL1 antibody

GABARAPL1, one of the proteins belonging to the GABARAP (GABA(A) receptor-associated protein) family, is highly expressed in the central nervous system and implicated in processes such as receptor and vesicle transport as well as autophagy. The proteins that make up the GABARAP family demonstrate conservation of their amino acid sequences and protein structures. In humans, GABARAPL1 shares 86% identity with GABARAP and 61% with GABARAPL2 (GATE-16) (1). GABARAPL1, or GABA(A) Receptor-Associated Protein Like 1, is a ubiquitin-like modifier that increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor. Involved in formation of autophagosomal vacuoles. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (2).

Product Categories/Family for anti-GABARAPL1 antibody

Neuroscience; Neurotransmitter Receptors; GABA Receptors; Ion Channels

References

1. Le Grand J.N., at al. (2011) Autophagy. 7(11): 1302-1307. 2. Tanida S., et al. (2006) FEBS J. 273(11): 2553-2562.

NCBI and Uniprot Product Information

• NCBI GI #: 13899219
• NCBI GeneID: 23710
• NCBI Accession #: NP_113600.1
• NCBI GenBank Nucleotide #: NM_031412.2
• UniProt Accession #: Q9H0R8; Q6FIE6; B4E0Y7
• Molecular Weight: Detects ~14-16kDa. Other observed bands at ~32kDa.
• NCBI Official Full Name: gamma-aminobutyric acid receptor-associated protein-like 1
• NCBI Official Synonym Full Names: GABA type A receptor associated protein like 1
• NCBI Official Symbol: GABARAPL1
• NCBI Official Synonym Symbols: ATG8; GEC1; APG8L; ATG8B; ATG8L; APG8-LIKE
• NCBI Protein Information: gamma-aminobutyric acid receptor-associated protein-like 1
• UniProt Protein Name: Gamma-aminobutyric acid receptor-associated protein-like 1
• Protein Family: Gamma-aminobutyric acid receptor-associated protein
• UniProt Gene Name: GABARAPL1
• UniProt Synonym Gene Names: GEC1; GEC-1
• UniProt Entry Name: GBRL1_HUMAN

Uniprot Description

GABARAPL1: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor. Belongs to the MAP1 LC3 family. Interacts with GABRG2 and beta-tubulin. Interacts with KOR-1.

Protein type: Adaptor/scaffold; Microtubule-binding; Vesicle; Ubiquitin-like modifier; Autophagy

Chromosomal Location of Human Ortholog: 12p13.2

Cellular Component: autophagic vacuole; cytoplasmic vesicle membrane; cytosol; dendrite cytoplasm; endoplasmic reticulum; Golgi apparatus; intracellular; microtubule; mitochondrion

Molecular Function: beta-tubulin binding; GABA receptor binding; protein binding; Tat protein binding; ubiquitin protein ligase binding

Biological Process: autophagic vacuole formation; cellular response to nitrogen starvation; macroautophagy; mitochondrion degradation