Rabbit GABARAP Polyclonal Antibody | anti-GABARAP antibody

GABARAP Polyclonal Antibody

Cat. Num. AAA3012736

• Gene Names: GABARAP; MM46; ATG8A; GABARAP-a
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: GABARAP; Polyclonal Antibody; GABARAP Polyclonal Antibody; FLC3B; HT004; ATG8A; FLC 3B; FLJ25768; GABA type A receptor associated protein; GABA(A) receptor associated protein; GABA(A) receptor-associated protein; GABARAP a; Gamma aminobutyric acid receptor associated protein; Gamma-aminobutyric acid receptor-associated protein; GBRAP; MGC120154; MGC120155; MM 46; MM46; anti-GABARAP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: GABARAP polyclonal antibody detects endogenous levels of GABARAP protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 117

• Applicable Applications for anti-GABARAP antibody: Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:500; IHC: 1:50-1:200; FC/FACS: 1:50-1:100
• Immunogen: Recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of GABARAP on mouse kidney (1) and rat liver (2) tissue lysate using anti-GABARAP antibody at 1/1,000 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-GABARAP antibody. Counter stained with hematoxylin.)

Related Product Information for anti-GABARAP antibody

In the central nervous system GABA functions as the main inhibitory transmitter by increasing a Cl-conductance that inhibits neuronal firing. GABA has been shown to activate both ionotropic (GABAA) and metabotropic (GABAB) receptors as well as a third class of receptors called GABAC. In addition to GABA receptors, several proteins have been identified as regulators of GABA function, including GAD65, GAD67, GABA transporters and GABARAP (GABAA receptor-associated protein). GABARAP associates with GABAA Rg2 to link GABAA receptors to the cytoskeleton. The GABARAP protein sequence is similar to light chain-3 of microtubule-associated proteins (MAPs) suggesting that it may be a type of MAP or a component of a MAP complex.

NCBI and Uniprot Product Information

• NCBI GI #: 6005764
• NCBI GeneID: 11337
• NCBI Accession #: NP_009209.1
• NCBI GenBank Nucleotide #: NP_009209.1
• UniProt Accession #: O95166; Q8R3R8; P60517
• Molecular Weight: 14kDa
• NCBI Official Full Name: gamma-aminobutyric acid receptor-associated protein
• NCBI Official Synonym Full Names: GABA type A receptor-associated protein
• NCBI Official Symbol: GABARAP
• NCBI Official Synonym Symbols: MM46; ATG8A; GABARAP-a
• NCBI Protein Information: gamma-aminobutyric acid receptor-associated protein
• UniProt Protein Name: Gamma-aminobutyric acid receptor-associated protein
• Protein Family: Gamma-aminobutyric acid receptor-associated protein
• UniProt Gene Name: GABARAP
• UniProt Synonym Gene Names: FLC3B
• UniProt Entry Name: GBRAP_HUMAN

NCBI Description

Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton. [provided by RefSeq, Jul 2008]

Uniprot Description

GABARAP: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. May play a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Belongs to the MAP1 LC3 family. Interacts with GPHN and NSF. Interacts with GABRG2, beta-tubulin and ULK1.

Protein type: Autophagy; Ubiquitin-like modifier; Adaptor/scaffold; Microtubule-binding; Vesicle

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: microtubule; smooth endoplasmic reticulum; lysosome; autophagic vacuole; cytosol; actin cytoskeleton; Golgi membrane; microtubule associated complex; extrinsic to membrane; perinuclear region of cytoplasm; plasma membrane; axoneme; cytoplasmic vesicle

Molecular Function: protein binding; microtubule binding; beta-tubulin binding; GABA receptor binding

Biological Process: synaptic transmission; induction of apoptosis via death domain receptors; mitochondrion degradation; microtubule cytoskeleton organization and biogenesis; protein targeting; autophagic vacuole formation