Western Blot (WB)
(Western Blot analysis in 293 cell line lysates (35ug/lane) using MBS6008427.This demonstrates MBS6008427 detected the C4orf31 protein (arrow).)
Rabbit anti-Human C4orf31 Polyclonal Antibody | anti-NDNF antibody
C4orf31, NT (NDNF, C4orf31, Protein NDNF, Neuron-derived neurotrophic factor)
Gene Names
NDNF; C4orf31
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
C4orf31; Polyclonal Antibody; NT (NDNF; Protein NDNF; Neuron-derived neurotrophic factor); Anti -C4orf31; anti-NDNF antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-NDNF antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Immunogen
C4orf31 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 87-117 amino acids from the N-terminal region of human C4orf31.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis in 293 cell line lysates (35ug/lane) using MBS6008427.This demonstrates MBS6008427 detected the C4orf31 protein (arrow).)
Western Blot (WB)
(Western Blot analysis in 293 cell line lysates (35ug/lane) using MBS6008427.This demonstrates MBS6008427 detected the C4orf31 protein (arrow).)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma using MBS6008427 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MBS6008427 for immunohistochemistry.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma using MBS6008427 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MBS6008427 for immunohistochemistry.)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of 293 cells using MBS6008427(right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of 293 cells using MBS6008427(right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-NDNF antibody
Promotes matrix assembly and cell adhesiveness (By similarity).
Product Categories/Family for anti-NDNF antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
64,673 Da
NCBI Official Full Name
Chromosome 4 open reading frame 31
NCBI Official Synonym Full Names
neuron-derived neurotrophic factor
NCBI Official Symbol
NDNF
NCBI Official Synonym Symbols
C4orf31
NCBI Protein Information
protein NDNF; fibronectin type-III domain-containing protein C4orf31
UniProt Protein Name
Protein NDNF
Protein Family
UniProt Gene Name
NDNF
UniProt Synonym Gene Names
C4orf31
UniProt Entry Name
NDNF_HUMAN
Uniprot Description
NDNF: Promotes matrix assembly and cell adhesiveness. Promotes neuron migration, growth and survival as well as neurite outgrowth.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 4q27
Cellular Component: extracellular matrix; extracellular region
Molecular Function: heparin binding; glycosaminoglycan binding
Biological Process: extracellular matrix organization and biogenesis; neuron migration; negative regulation of neuron apoptosis; cell growth; peptide cross-linking via chondroitin 4-sulfate glycosaminoglycan
Research Articles on NDNF
Similar Products
Product Notes
The NDNF ndnf (Catalog #AAA6008427) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The C4orf31, NT (NDNF, C4orf31, Protein NDNF, Neuron-derived neurotrophic factor) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's C4orf31 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the NDNF ndnf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C4orf31, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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