Rabbit GAB1 Polyclonal Antibody | anti-GAB1 antibody

Rabbit anti GAB1 (pTyr627)

Cat. Num. AAA225258

• Reactivity: Human, Mouse (Expected from Sequence)
• Applications: Western Blot
• Purity: Purified whole serum by affinity chromatography.
• Synonyms: GAB1; Polyclonal Antibody; Rabbit anti GAB1 (pTyr627); GAB1 (pTyr627) Antibody; anti-GAB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse (Expected from Sequence)
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified whole serum by affinity chromatography.
• Form/Format: Liquid in phosphate buffered saline with 0.02% sodium azide, 50% glycerol.
• Sequence Length: 694

• Applicable Applications for anti-GAB1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Phospho specific-peptide corresponding to residues surrounding tyrosine 627 of human GAB1
• Preperation: Antiserum to GAB1 (pTyr627) was raised by repeated immunization of rabbits with highly purified antigen.
• Preparation and Storage: Store at -20 degree C.; Storage in frost-free freezers is not recommended. This product should be stored undiluted.; Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch

Western Blot (WB)

(Western blot analysis of extracts from HEK-293 cells untreated or treated with EGF using Rabbit anti GAB1 (pTyr627) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with EGF (100ng/ml) for 30 minutes)

Related Product Information for anti-GAB1 antibody

Rabbit anti GAB1 (pTyr627) antibody recognizes GRB2-associated-binding protein 1, also known as GAB1, GRB2-associated binder 1 or Growth factor receptor bound protein 2-associated protein 1 when phosphorylated at tyrosine 627. GAB1 is a docking protein capable of interactions with several different proteins particularly the activation of receptor tyrosine kinase signaling. Phosphorylation of GAB1 at tyrosine 627 leads to SHP2 recruitment and subsequent activation of MAPK signaling.

NCBI and Uniprot Product Information

• NCBI GI #: 90180201
• NCBI GeneID: 2549
• UniProt Accession #: Q13480; Q4W5G2; Q6P1W2; A8K152
• Molecular Weight: 80,005 Da
• NCBI Official Full Name: GRB2-associated-binding protein 1
• NCBI Official Synonym Full Names: GRB2 associated binding protein 1
• NCBI Official Symbol: GAB1
• NCBI Protein Information: GRB2-associated-binding protein 1
• UniProt Protein Name: GRB2-associated-binding protein 1
• Protein Family: GPI transamidase component
• UniProt Gene Name: GAB1

NCBI Description

The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

Adapter protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases. Plays a role in FGFR1 signaling. Probably involved in signaling by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR).