Mouse Alkaline Phosphatase (ALP) ELISA Kit | ALP elisa kit

Mouse Alkaline Phosphatase (ALP) ELISA Kit

Cat. Num. AAA2703334

• Gene Names: ALPL; HOPS; TNAP; APTNAP; TNSALP; AP-TNAP
• Reactivity: Mouse
• Synonyms: Alkaline Phosphatase (ALP); Mouse Alkaline Phosphatase (ALP) ELISA Kit; AKP; ALKP; Basic Phosphatase; ALP elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Alkaline Phosphatase (ALP).; No significant cross-reactivity or interference between Alkaline Phosphatase (ALP) and analogues was observed.
• Sequence Length: 469

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.059ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alkaline Phosphatase (ALP) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alkaline Phosphatase (ALP) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ALP elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alkaline Phosphatase (ALP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alkaline Phosphatase (ALP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alkaline Phosphatase (ALP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alkaline Phosphatase (ALP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 178462
• NCBI GeneID: 249
• UniProt Accession #: P05186; O75090; Q2TAI7; Q59EJ7; Q5BKZ5; Q5VTG5; Q6NZI8; Q8WU32; A1A4E7; B2RMP8; B7Z387; B7Z4Y6
• Molecular Weight: 51,045 Da
• NCBI Official Full Name: alkaline phosphatase
• NCBI Official Synonym Full Names: alkaline phosphatase, liver/bone/kidney
• NCBI Official Symbol: ALPL
• NCBI Official Synonym Symbols: HOPS; TNAP; APTNAP; TNSALP; AP-TNAP
• NCBI Protein Information: alkaline phosphatase, tissue-nonspecific isozyme
• UniProt Protein Name: Alkaline phosphatase, tissue-nonspecific isozyme
• Protein Family: Alkaline proteinase
• UniProt Gene Name: ALPL
• UniProt Synonym Gene Names: AP-TNAP; TNSALP

NCBI Description

This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects. [provided by RefSeq, Oct 2015]

Uniprot Description

This isozyme may play a role in skeletal mineralization.