Rabbit anti-Human G3BP1 Polyclonal Antibody | anti-G3BP1 antibody

G3BP1, CT (G3BP1, G3BP, Ras GTPase-activating protein-binding protein 1, ATP-dependent DNA helicase VIII, GAP SH3 domain-binding protein 1) (Azide free) (HRP)

Cat. Num. AAA6300646

• Gene Names: G3BP1; G3BP; HDH-VIII
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: G3BP1; Polyclonal Antibody; CT (G3BP1; G3BP; Ras GTPase-activating protein-binding protein 1; ATP-dependent DNA helicase VIII; GAP SH3 domain-binding protein 1) (Azide free) (HRP); GAP SH3 domain-binding protein 1; anti-G3BP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human G3BP1.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-G3BP1 antibody: Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 401-430 in the C-terminal region of human G3BP1. Species sequence homology: bovine, mouse
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(G3BP1 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the G3BP1 antibody detected the G3BP1 protein (arrow).)

Immunohistochemistry (IHC)

(G3BP1 Antibody (C-term) (AP13785b)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of G3BP1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-G3BP1 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

1. Gao, X., et al. FEBS Lett. 584(16):3525-3532(2010). 2. Ortega, A.D., et al. J. Cell. Sci. 123 (PT 16), 2685-2696 (2010). 3. Hinton, S.D., et al. Biochem. J. 427(3):349-357(2010). 4. Shim, J.H., et al. Cancer Prev Res (Phila) 3(5):670-679(2010). 5. Zhang, H.Z., et al. World J. Gastroenterol. 13(30):4126-4130(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 38327552
• NCBI GeneID: 10146
• NCBI Accession #: NP_938405.1; NP_005745.1
• NCBI GenBank Nucleotide #: NM_198395.1
• UniProt Accession #: Q13283; Q5HYE9
• Molecular Weight: 13,874 Da
• NCBI Official Full Name: ras GTPase-activating protein-binding protein 1
• NCBI Official Synonym Full Names: G3BP stress granule assembly factor 1
• NCBI Official Symbol: G3BP1
• NCBI Official Synonym Symbols: G3BP; HDH-VIII
• NCBI Protein Information: ras GTPase-activating protein-binding protein 1
• UniProt Protein Name: Ras GTPase-activating protein-binding protein 1
• Protein Family: Ras GTPase-activating protein-binding protein
• UniProt Gene Name: G3BP1
• UniProt Synonym Gene Names: G3BP; G3BP-1; hDH VIII
• UniProt Entry Name: G3BP1_HUMAN

NCBI Description

This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

G3BP-1: an hnRNA-binding protein and endoribonuclease that participates in the Ras signal transduction pathway. A regulated effector of stress granule (SG) assembly. SGs are involved in mRNA sorting in the storing of untranslated mRNAs. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA. Binds to the SH3 domain of Ras GTPase-activating protein (RasGAP) in proliferating cells but not in quiescent cells. Cytoplasmic in proliferating cells, can be recruited to the plasma membrane in exponentially growing cells. Cytosolic and partially nuclear in resting cells. Recruited to SGs upon either arsenite or high temperature treatment. RasGAP-dependent phosphorylation of S149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. S149 phosphorylation induces partial nuclear localization. A component of a TAU mRNP complex, Interacts with USP10, and may regulate it.

Protein type: EC 3.6.4.12; RNA-binding; Helicase; Adaptor/scaffold; EC 3.6.4.13

Chromosomal Location of Human Ortholog: 5q33.1

Cellular Component: focal adhesion; stress granule; cytoplasm; plasma membrane; cytosol; nucleus

Molecular Function: mRNA binding; ATP-dependent DNA helicase activity; protein binding; DNA binding; endonuclease activity; ATP-dependent RNA helicase activity; ATP binding

Biological Process: transport; metabolic process; Ras protein signal transduction; DNA duplex unwinding