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Western Blot (WB) (Western Blot: Sample: Lane1: Human Placenta Tissue; Lane2: Human PC-3 Cells; Lane3: Human MCF7 Cells.)

Rabbit anti-Human Ki-67 Protein (Ki67P) Polyclonal Antibody | anti-Ki67P antibody

Polyclonal Antibody to Ki-67 Protein (Ki67P)

Gene Names
MKI67; KIA; MIB-; MIB-1; PPP1R105
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Ki-67 Protein (Ki67P); Polyclonal Antibody; Polyclonal Antibody to Ki-67 Protein (Ki67P); anti-Ki67P antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against Ki67P. It has been selected for its ability to recognize Ki67P in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
0.58mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- GIS LRSRRQNKTE AEQQITEVFV LAERIEINRN EKKPMKTSPE MDIQNPDDGA RKPIPRDKVT ENKRCLRSAR QNESSQPKVA EESGGQKSAK VLMQNQKGKG EAGNSDSMCL RSRKTKSQPA ASTLESKSVQ RVTRSVKRCA ENPKK
Sequence Length
2938
Applicable Applications for anti-Ki67P antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant Ki67P (Gly3088~Lys3235) expressed in E.coli.
Cross Reactivity
Human, Mouse
Conjugated Antibody
The Cy3 conjugated antibody version of this item is also available as catalog #MBS2062502
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Lane1: Human Placenta Tissue; Lane2: Human PC-3 Cells; Lane3: Human MCF7 Cells.)

Western Blot (WB) (Western Blot: Sample: Lane1: Human Placenta Tissue; Lane2: Human PC-3 Cells; Lane3: Human MCF7 Cells.)

Western Blot (WB)

(Western Blot: Sample: Recombinant Ki67P, Human.)

Western Blot (WB) (Western Blot: Sample: Recombinant Ki67P, Human.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin-embedded esophagus cancer tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin-embedded esophagus cancer tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
319,444 Da
NCBI Official Full Name
proliferation marker protein Ki-67 isoform 2
NCBI Official Synonym Full Names
marker of proliferation Ki-67
NCBI Official Symbol
MKI67
NCBI Official Synonym Symbols
KIA; MIB-; MIB-1; PPP1R105
NCBI Protein Information
proliferation marker protein Ki-67; antigen KI-67
UniProt Protein Name
Proliferation marker protein Ki-67
UniProt Gene Name
MKI67
UniProt Synonym Gene Names
Antigen KI-67Curated; Antigen Ki67Curated

NCBI Description

This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]

Uniprot Description

KI-67: a protein that may be a marker of proliferating cells, involved in chromatin compaction. Its expression is altered in many tumor types including osteosarcomas, histiocytomas, prostate, breast and esophageal cancers. Mutated in colon, cervical and lung cancers.

Protein type: Cell cycle regulation; Nucleolus

Chromosomal Location of Human Ortholog: 10q26.2

Cellular Component: condensed chromosome; membrane; nuclear body; nucleolus; nucleus

Molecular Function: ATP binding; DNA binding; protein binding; protein C-terminus binding; RNA binding

Biological Process: cell cycle; cell proliferation; regulation of chromatin organization; regulation of chromosome segregation; regulation of mitotic nuclear division

Research Articles on Ki67P

Similar Products

Product Notes

The Ki67P mki67 (Catalog #AAA2002592) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Ki-67 Protein (Ki67P) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Ki-67 Protein (Ki67P) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the Ki67P mki67 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- GIS LRSRRQNKTE AEQQITEVFV LAERIEINRN EKKPMKTSPE MDIQNPDDGA RKPIPRDKVT ENKRCLRSAR QNESSQPKVA EESGGQKSAK VLMQNQKGKG EAGNSDSMCL RSRKTKSQPA ASTLESKSVQ RVTRSVKRCA ENPKK. It is sometimes possible for the material contained within the vial of "Ki-67 Protein (Ki67P), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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