Western Blot (WB)
(Western blot analysis of G15 expression in RT4 (A), NIH3T3 (B) whole cell lysates.)
Rabbit G15 Polyclonal Antibody | anti-RBM5 antibody
Anti-G15 Antibody
Western Blot (WB)
(Western blot analysis of G15 expression in RT4 (A), NIH3T3 (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of G15 expression in RT4 (A), NIH3T3 (B) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of G15 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of G15 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
This gene is a candidate tumor suppressor gene which encodes a nuclear RNA binding protein that is a component of the spliceosome A complex. The encoded protein plays a role in the induction of cell cycle arrest and apoptosis through pre-mRNA splicing of multiple target genes including the tumor suppressor protein p53. This gene is located within the tumor suppressor region 3p21.3, and may play a role in the inhibition of tumor transformation and progression of several malignancies including lung cancer. [provided by RefSeq, Oct 2011]
Uniprot Description
RBM5: Component of the spliceosome A complex. Regulates alternative splicing of a number of mRNAs. May modulate splice site pairing after recruitment of the U1 and U2 snRNPs to the 5' and 3' splice sites of the intron. May both positively and negatively regulate aopotosis by regulating the alternative splicing of several genes involved in this process, including FAS and CASP2/caspase-2. In the case of FAS, promotes exclusion of exon 6 thereby producing a soluble form of FAS that inhibits apoptosis. In the case of CASP2/caspase-2, promotes exclusion of exon 9 thereby producing a catalytically active form of CASP2/Caspase-2 that induces apoptosis. Belongs to the RBM5/RBM10 family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: RNA-binding; RNA processing; RNA splicing; Apoptosis; C2H2-type zinc finger protein; Cell cycle regulation
Chromosomal Location of Human Ortholog: 3p21.3
Cellular Component: nucleoplasm; spliceosome; nucleus
Molecular Function: mRNA binding; protein binding; DNA binding; zinc ion binding; RNA binding; nucleotide binding
Biological Process: RNA processing; nuclear mRNA splicing, via spliceosome; negative regulation of cell proliferation; apoptosis; positive regulation of apoptosis; RNA splicing; spliceosome assembly; gene expression; regulation of alternative nuclear mRNA splicing, via spliceosome
Research Articles on RBM5
Similar Products
Product Notes
The RBM5 rbm5 (Catalog #AAA8226529) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-G15 Antibody reacts with Human, Mouse, Rat, Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's G15 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB (1/500 - 1/1000), IHC (1/100 - 1/200). Researchers should empirically determine the suitability of the RBM5 rbm5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "G15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
