Goat Defensin 1, Neutrophil ELISA Kit | DEFA1 elisa kit

Goat Defensin 1, Neutrophil ELISA Kit

Cat. Num. AAA738740

• Gene Names: DEFA1; HP1; MRS; DEF1; HP-1; DEFA2; HNP-1
• Reactivity: Goat
• Synonyms: Defensin 1; Neutrophil; Goat Defensin 1; Neutrophil ELISA Kit; DEFA1 elisa kit

• Reactivity: Goat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Sensitivity: 1.0 ng/mL.
• Intended Uses: This LH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat LH. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DEFA1 elisa kit

Principle of the Assay: LH ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for LH. Standards or samples are then added to the microtiter plate wells and LH if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of LH present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for LH are added to each well to "sandwich" the LH immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain LH and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LH concentration in each sample is interpolated from this standard curve.

Product Categories/Family for DEFA1 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 50057842
• NCBI GeneID: 1667
• Molecular Weight: 10,201 Da
• NCBI Official Full Name: defensin, alpha 1, myeloid-related sequence
• NCBI Official Synonym Full Names: defensin, alpha 1
• NCBI Official Symbol: DEFA1
• NCBI Official Synonym Symbols: HP1; MRS; DEF1; HP-1; DEFA2; HNP-1
• NCBI Protein Information: neutrophil defensin 1; defensin, alpha 2; myeloid-related sequence; defensin, alpha 1, myeloid-related sequence
• UniProt Protein Name: Neutrophil defensin 1
• Protein Family: Alpha-defensin
• UniProt Gene Name: DEFA1
• UniProt Synonym Gene Names: DEF1; DEFA2; MRS; DEFA1B; HP-1; HP1; HP-2; HP2
• UniProt Entry Name: DEF1_HUMAN

NCBI Description

Defensins are a family of microbicidal and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation. [provided by RefSeq, May 2010]

Uniprot Description

defensin, alpha 1: Defensin 1 and defensin 2 have antibacterial, fungicide and antiviral activities. Has antimicrobial activity against Gram- negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. Belongs to the alpha-defensin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: extracellular matrix; extracellular space; Golgi lumen; extracellular region

Biological Process: defense response to Gram-positive bacterium; estrogen receptor signaling pathway; innate immune response in mucosa; killing of cells of another organism; antibacterial humoral response; innate immune response; immune response; chemotaxis; defense response to fungus; defense response to virus