Rabbit FZD6 / Frizzled 6 Polyclonal Antibody | anti-FZD6 antibody

Rabbit Polyclonal to Human FZD6 / Frizzled 6

Cat. Num. AAA247381

• Gene Names: FZD6; FZ6; FZ-6; HFZ6; NDNC10
• Reactivity: Gibbon, Gorilla, Human, Monkey, Orangutan; Predicted Reactivity: Mouse, Bovine, Dog, Horse, Pig, Rabbit (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Immunocytochemistry
• Purity: Immunoaffinity Purified
• Synonyms: FZD6 / Frizzled 6; Polyclonal Antibody; Rabbit Polyclonal to Human FZD6 / Frizzled 6; Anti-FZD6 / Frizzled 6 Antibody (N-Terminus) IHC-plus; FZD6; Frizzled family receptor 6; FZ-6; HFZ6; Frizzled 6; Frizzled-6; Frizzled homolog 6; FZ6; NDNC10; Human FZD6; anti-FZD6 antibody

• Host: Rabbit
• Reactivity: Gibbon, Gorilla, Human, Monkey, Orangutan; Predicted Reactivity: Mouse, Bovine, Dog, Horse, Pig, Rabbit (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human Frizzled-6. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except FZD3 (50%).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 706

• Applicable Applications for anti-FZD6 antibody: Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC)
• Application Notes: ICC (3 - 6 ug/ml), IHC-P (6 - 14 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic 16 amino acid peptide from N-terminal extracellular domain of human Frizzled-6. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%).
• Immunogen Type: Synthetic peptide
• Immunogen: FZD6 / Frizzled 6 antibody was raised against synthetic 16 amino acid peptide from N-terminal extracellular domain of human Frizzled-6. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%); Mouse, Panda, Bovine, Dog, Horse, Rabbit, Pig (94%); Rat, Opossum, Turkey, Chicken (88%); Elephant, Bat, Lizard (81%).
• Antigen Modification: N-Terminus
• Preparation and Storage: Long term: -70 degree C; Short term: +4 degree C

Immunohistochemistry (IHC)

(Anti-FZD6 / Frizzled 6 antibody IHC of human Lung, Non-Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-Frizzled-6 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-FZD6 / Frizzled 6 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Transfection

(Anti-Frizzled-6 antibody immunocytochemistry (ICC) staining of HEK293 human embryonic kidney cells transfected with Frizzled-6.)

Transfection Control

(Anti-Frizzled-6 antibody immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.)

Related Product Information for anti-FZD6 antibody

FZD6 is a Frizzled Receptor for Wnt signaling proteins involved in patterning during development. Contrary to other Frizzled proteins, FZD6 does not contain a C-terminal PDZ domain-binding motif. FZD6 is widely expressed. The Drosophila homolog activates Dishevelled.

Product Categories/Family for anti-FZD6 antibody

Family: GPCR; Subfamily: Frizzled

NCBI and Uniprot Product Information

• NCBI GI #: 34734079
• NCBI GeneID: 8323
• NCBI Accession #: NP_003497.2
• NCBI GenBank Nucleotide #: NM_003506.3
• UniProt Accession #: O60353; Q6N0A5; Q6P9C3; Q8WXR9; B4DRN0
• Molecular Weight: 75,579 Da
• NCBI Official Full Name: frizzled-6 isoform a
• NCBI Official Synonym Full Names: frizzled class receptor 6
• NCBI Official Symbol: FZD6
• NCBI Official Synonym Symbols: FZ6; FZ-6; HFZ6; NDNC10
• NCBI Protein Information: frizzled-6; frizzled homolog 6; frizzled family receptor 6; seven transmembrane helix receptor; frizzled 6, seven transmembrane spanning receptor
• UniProt Protein Name: Frizzled-6
• Protein Family: Frizzled
• UniProt Gene Name: FZD6
• UniProt Synonym Gene Names: Fz-6; hFz6
• UniProt Entry Name: FZD6_HUMAN

NCBI Description

This gene represents a member of the 'frizzled' gene family, which encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The protein encoded by this family member contains a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, and seven transmembrane domains, but unlike other family members, this protein does not contain a C-terminal PDZ domain-binding motif. This protein functions as a negative regulator of the canonical Wnt/beta-catenin signaling cascade, thereby inhibiting the processes that trigger oncogenic transformation, cell proliferation, and inhibition of apoptosis. Alternative splicing results in multiple transcript variants, some of which do not encode a protein with a predicted signal peptide.[provided by RefSeq, Aug 2011]

Uniprot Description

FZD6: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Detected in adult heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, thymus, prostate, testis, ovary, small intestine and colon. In the fetus, expressed in brain, lung, liver and kidney. Belongs to the G-protein coupled receptor Fz/Smo family.

Protein type: GPCR, Fz/Smo family; Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR

Chromosomal Location of Human Ortholog: 8q22.3-q23.1

Cellular Component: cell surface; cytoplasmic vesicle membrane; apicolateral plasma membrane; integral to plasma membrane; apical plasma membrane; plasma membrane; integral to membrane

Molecular Function: G-protein coupled receptor activity; Wnt-protein binding; protein binding; Wnt receptor activity; ubiquitin protein ligase binding

Biological Process: platelet activation; G-protein coupled receptor protein signaling pathway; inner ear morphogenesis; hair follicle development; Wnt receptor signaling pathway, planar cell polarity pathway; negative regulation of transcription factor activity; neural tube closure; establishment of planar polarity; cell proliferation in midbrain

Disease: Nail Disorder, Nonsyndromic Congenital, 10