Rabbit Fyn Polyclonal Antibody | anti-Fyn antibody

Fyn (Phospho-Tyr530) Antibody

Cat. Num. AAA9403240

• Gene Names: FYN; SLK; SYN; p59-FYN
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: Fyn; Polyclonal Antibody; Fyn (Phospho-Tyr530) Antibody; SLK; SYN; p59-FYN; anti-Fyn antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of Fyn only when phosphorylated at Tyrosine 530.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 537

• Applicable Applications for anti-Fyn antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500~1:1000
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of Tyrosine 530
• Modification: Phospho-Tyr530
• Target Name: Fyn
• Preparation and Storage: Store at -20 degree C/1 year

Western Blot (WB)

(Western blot analysis of extracts from Mouse brain tissue using Fyn (Phospho-Tyr530) Antibody.The lane on the left is treated with the antigen-specific peptide.)

Related Product Information for anti-Fyn antibody

Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1.

Product Categories/Family for anti-Fyn antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4503823
• NCBI GeneID: 2534
• NCBI Accession #: NP_002028.1
• NCBI GenBank Nucleotide #: NM_002037.5
• UniProt Accession #: P06241; Q16248; Q5R3A6; Q5R3A7; Q8N5D7; B5BU57; E1P557; H0UI48
• Molecular Weight: 54,513 Da
• NCBI Official Full Name: tyrosine-protein kinase Fyn isoform a
• NCBI Official Synonym Full Names: FYN proto-oncogene, Src family tyrosine kinase
• NCBI Official Symbol: FYN
• NCBI Official Synonym Symbols: SLK; SYN; p59-FYN
• NCBI Protein Information: tyrosine-protein kinase Fyn
• UniProt Protein Name: Tyrosine-protein kinase Fyn
• Protein Family: FYN-binding protein
• UniProt Gene Name: FYN
• UniProt Synonym Gene Names: SLK
• UniProt Entry Name: FYN_HUMAN

NCBI Description

This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. [provided by RefSeq, Jul 2008]

Uniprot Description

Fyn: a tyrosine kinase of the Src family. Implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC. Associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Three alternatively spliced isoforms have been described. Isoform 2 shows a greater ability to mobilize cytoplasmic calcium than isoform 1. Induced expression aids in cellular transformation and xenograft metastasis. In squamous cell carcinoma, Fyn transduces signals from EGFR and Src and is required for cell migration and invasiveness. Activity linked to migration in a murine melanoma model. Appears to block late stage development of neuroblastoma. Mouse knockout deficient in kindling response, a model for human epilepsy.

Protein type: Oncoprotein; Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; Kinase, protein; Protein kinase, TK; TK group; Src family

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: extrinsic to internal side of plasma membrane; mitochondrion; postsynaptic density; plasma membrane; actin filament; nucleus; cytosol; endosome; lipid raft

Molecular Function: tubulin binding; CD8 receptor binding; protein binding; G-protein-coupled receptor binding; peptide hormone receptor binding; ephrin receptor binding; metal ion binding; protein-tyrosine kinase activity; T cell receptor binding; non-membrane spanning protein tyrosine kinase activity; phosphoinositide 3-kinase binding; glycoprotein binding; CD4 receptor binding; ATP binding

Biological Process: axon guidance; central nervous system development; T cell activation; nerve growth factor receptor signaling pathway; viral reproduction; neuron migration; T cell receptor signaling pathway; protein amino acid phosphorylation; regulation of apoptosis; regulation of cell shape; calcium ion transport; forebrain development; ephrin receptor signaling pathway; feeding behavior; negative regulation of neuron apoptosis; cell differentiation; epidermal growth factor receptor signaling pathway; response to drug; platelet activation; cell migration; positive regulation of I-kappaB kinase/NF-kappaB cascade; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; dendrite morphogenesis; activated T cell proliferation; learning; regulation of defense response to virus by virus; regulation of cell proliferation; positive regulation of phosphoinositide 3-kinase cascade; detection of mechanical stimulus involved in sensory perception of pain; response to ethanol; T cell costimulation; innate immune response; negative regulation of protein catabolic process; vascular endothelial growth factor receptor signaling pathway; blood coagulation; leukocyte migration; transmembrane receptor protein tyrosine kinase signaling pathway