Western Blot (WB)
((1ug/ml) staining of mouse heart lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Goat FXY2 Polyclonal Antibody
FXY2 (MID2, Probable E3 Ubiquitin-protein Ligase MID2, Midin-2, Midline Defect 2, Midline-2, RING Finger Protein 60, Tripartite Motif-containing Protein 1, RNF60, TRIM1)
Reactivity
Bovine, Canine, Human, Mouse, Porcine, Rat
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
FXY2; Polyclonal Antibody; FXY2 (MID2; Probable E3 Ubiquitin-protein Ligase MID2; Midin-2; Midline Defect 2; Midline-2; RING Finger Protein 60; Tripartite Motif-containing Protein 1; RNF60; TRIM1); Anti -FXY2 (MID2; anti-FXY2 antibody
Host
Goat
Reactivity
Bovine, Canine, Human, Mouse, Porcine, Rat
Clonality
Polyclonal
Specificity
Recognizes human MID2. Species Crossreactivity
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Applicable Applications for anti-FXY2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: ELISA: 1:32,000
Western Blot: 1-3ug/ml, observed in mouse heart lysates on ~80kD bands
Immunohistochemistry (Paraffin): 5-10ug/ml
Dilution: ELISA: 1:32,000
Western Blot: 1-3ug/ml, observed in mouse heart lysates on ~80kD bands
Immunohistochemistry (Paraffin): 5-10ug/ml
Immunogen
Synthetic peptide corresponding to QESPYVSGMKTCH, from MID2, at C-terminal (NP_036348.2, NP_438112.2).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
((1ug/ml) staining of mouse heart lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of mouse heart lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Related Product Information for anti-FXY2 antibody
MID2 is a member of the tripartite motif (TRIM) family. The MID motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Its function has not been identified. Alternate splicing of the gene encoding MID2 results in two transcript variants encoding different isoforms.
Product Categories/Family for anti-FXY2 antibody
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Product Notes
The FXY2 (Catalog #AAA648850) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The FXY2 (MID2, Probable E3 Ubiquitin-protein Ligase MID2, Midin-2, Midline Defect 2, Midline-2, RING Finger Protein 60, Tripartite Motif-containing Protein 1, RNF60, TRIM1) reacts with Bovine, Canine, Human, Mouse, Porcine, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's FXY2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot and Immunohistochemistry. Dilution: ELISA: 1:32,000 Western Blot: 1-3ug/ml, observed in mouse heart lysates on ~80kD bands Immunohistochemistry (Paraffin): 5-10ug/ml. Researchers should empirically determine the suitability of the FXY2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FXY2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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