Immunohistochemistry (IHC)
(MBS9603630 at 1/100 staining Human pancreas cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit FXR1 Polyclonal Antibody | anti-FXR1 antibody
FXR1 Antibody
Gene Names
FXR1; FXR1P
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
FXR1; Polyclonal Antibody; FXR1 Antibody; Fragile X mental retardation autosomal homolog 1; Fragile X mental retardation syndrome related protein 1; Fragile X mental retardation syndrome-related protein 1; FXR1_HUMAN; FXR1P; hFXR1p; anti-FXR1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
FXR1 antibody detects endogenous levels of FXR1.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
539
Applicable Applications for anti-FXR1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human FXR1.
Subcellular Location
Cytoplasm.
Tissue Specificity
Expressed in all tissues examined including heart, brain, kidney and testis.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Zebrafish (83%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (92%), Xenopus (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9603630 at 1/100 staining Human pancreas cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9603630 at 1/100 staining Human pancreas cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using FXR1 antibody.)
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using FXR1 antibody.)
Related Product Information for anti-FXR1 antibody
Function: RNA-binding protein required for embryonic and postnatal development of muscle tissue. May regulate intracellular transport and local translation of certain mRNAs (By similarity).
Subunit Structure: Interacts with FMR1 (PubMed:8668200, PubMed:7489725, PubMed:11157796). Interacts with FRX2 (PubMed:7489725). Interacts with TDRD3 (PubMed:18664458). Interacts with HABP4 (PubMed:21771594). Interacts with CYFIP2 but not with CYFIP1 (By similarity).
Post-translational Modifications: Arg-445 is dimethylated, probably to asymmetric dimethylarginine.
Similarity: The tandem Agenet-like domains preferentially recognize trimethylated histone peptides. Belongs to the FMR1 family.
Subunit Structure: Interacts with FMR1 (PubMed:8668200, PubMed:7489725, PubMed:11157796). Interacts with FRX2 (PubMed:7489725). Interacts with TDRD3 (PubMed:18664458). Interacts with HABP4 (PubMed:21771594). Interacts with CYFIP2 but not with CYFIP1 (By similarity).
Post-translational Modifications: Arg-445 is dimethylated, probably to asymmetric dimethylarginine.
Similarity: The tandem Agenet-like domains preferentially recognize trimethylated histone peptides. Belongs to the FMR1 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 60-80 kDa
Predicted: 70 kDa
Predicted: 70 kDa
NCBI Official Full Name
fragile X mental retardation syndrome-related protein 1 isoform b
NCBI Official Synonym Full Names
FMR1 autosomal homolog 1
NCBI Official Symbol
FXR1
NCBI Official Synonym Symbols
FXR1P
NCBI Protein Information
fragile X mental retardation syndrome-related protein 1
UniProt Protein Name
Fragile X mental retardation syndrome-related protein 1
Protein Family
UniProt Gene Name
FXR1
UniProt Synonym Gene Names
hFXR1p
NCBI Description
The protein encoded by this gene is an RNA binding protein that interacts with the functionally-similar proteins FMR1 and FXR2. These proteins shuttle between the nucleus and cytoplasm and associate with polyribosomes, predominantly with the 60S ribosomal subunit. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
RNA-binding protein required for embryonic and postnatal development of muscle tissue. May regulate intracellular transport and local translation of certain mRNAs ().
Research Articles on FXR1
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Product Notes
The FXR1 fxr1 (Catalog #AAA9603630) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FXR1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's FXR1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the FXR1 fxr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FXR1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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