Rabbit anti-Human FUSIP1 Polyclonal Antibody | anti-SRSF10 antibody

FUSIP1 (FUS-interacting Protein (Serine-arginine Rich) 1, FUS Interacting Serine Arginine Rich Protein 1, 40kD SR-repressor Protein, FUSIP2, NSSR, OTTHUMP00000015774, Serine Arginine Repressor Protein 40kD, SFRS13, Splicing Factor SRp38, SRp38, SRp40, TLS

Cat. Num. AAA620802

• Gene Names: SRSF10; NSSR; TASR; SRp38; TASR1; TASR2; FUSIP1; FUSIP2; SFRS13; SRrp40; SFRS13A; FLJ30749; FLJ43846; DKFZp686H0644
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: FUSIP1; Polyclonal Antibody; FUSIP1 (FUS-interacting Protein (Serine-arginine Rich) 1; FUS Interacting Serine Arginine Rich Protein 1; 40kD SR-repressor Protein; FUSIP2; NSSR; OTTHUMP00000015774; Serine Arginine Repressor Protein 40kD; SFRS13; Splicing Factor SRp38; SRp38; SRp40; TLS; Anti -FUSIP1 (FUS-interacting Protein (Serine-arginine Rich) 1; anti-SRSF10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FUSIP1. Species sequence homology: 100% to mouse FUSIP1 isoform 3.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris-citrate/phosphate, pH 7-8, 0.01% sodium azide.

• Applicable Applications for anti-SRSF10 antibody: Western Blot (WB)
• Application Notes: Suitable for use Western Blot.; Dilution: Western Blot: 0.5 mg/ml,
• Immunogen: Synthetic peptide corresponding to an internal portion of the human protein (within residues 100-200). [O75494]
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 3 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

(Detection of FUSIP1, Isoform 3, in HepG2 whole cell lysates using NB110-93598.)

Related Product Information for anti-SRSF10 antibody

FUSIP1 is a member of the serine-arginine (SR) family of proteins, which is involved in constitutive and regulated RNA splicing. Members of this family are characterized by N-terminal RNP1 and RNP2 motifs, which are required for binding to RNA, and multiple C-terminal SR/RS repeats, which are important in mediating association with other cellular proteins. FUSIP1 can influence splice site selection of adenovirus E1A pre-mRNA. It interacts with the oncoprotein TLS, and abrogates the influence of TLS on E1A pre-mRNA splicing. Alternative splicing of this gene results in at least two transcript variants encoding different isoforms. This antibody is specific for isoform 3 of FUSIP1.

Product Categories/Family for anti-SRSF10 antibody

Antibodies; Abs to Splicing Factors

NCBI and Uniprot Product Information

• NCBI GI #: 55961042
• NCBI GeneID: 10772
• UniProt Accession #: O75494; O60572; Q5JRH9; Q5JRI0; Q5JRI2; Q5JRI4; Q96G09; Q96P17; A6NFM6; A6NI42; A6NIU7; Q53GD7; Q5JRI1; Q5JRI3; Q6IQ42
• Molecular Weight: 31,301 Da
• NCBI Official Full Name: FUS interacting protein (serine/arginine-rich) 1
• NCBI Official Synonym Full Names: serine/arginine-rich splicing factor 10
• NCBI Official Symbol: SRSF10
• NCBI Official Synonym Symbols: NSSR; TASR; SRp38; TASR1; TASR2; FUSIP1; FUSIP2; SFRS13; SRrp40; SFRS13A; FLJ30749; FLJ43846; DKFZp686H0644
• NCBI Protein Information: serine/arginine-rich splicing factor 10; OTTHUMP00000015773; OTTHUMP00000015774; OTTHUMP00000015775; OTTHUMP00000015776; OTTHUMP00000015777; OTTHUMP00000015778; SR splicing factor 10; splicing factor SRp38; TLS-associated SR protein; neural-salient SR protein; 40 kDa SR-repressor protein; TLS-associated protein TASR; TLS-associated protein with SR repeats; TLS-associated serine-arginine protein 1; TLS-associated serine-arginine protein 2; splicing factor, arginine/serine-rich 13; splicing factor, arginine/serine-rich 13A; serine-arginine repressor protein (40 kDa); TLS-associated protein with Ser-Arg repeats; FUS-interacting serine-arginine-rich protein 1; FUS interacting protein (serine-arginine rich) 1; FUS-interacting protein (serine-arginine rich) 2
• UniProt Protein Name: Serine/arginine-rich splicing factor 10
• Protein Family: Serine/arginine-rich splicing factor
• UniProt Gene Name: SRSF10
• UniProt Synonym Gene Names: FUSIP1; FUSIP2; SFRS13A; TASR
• UniProt Entry Name: SRS10_HUMAN

NCBI Description

This gene product is a member of the serine-arginine (SR) family of proteins, which is involved in constitutive and regulated RNA splicing. Members of this family are characterized by N-terminal RNP1 and RNP2 motifs, which are required for binding to RNA, and multiple C-terminal SR/RS repeats, which are important in mediating association with other cellular proteins. This protein can influence splice site selection of adenovirus E1A pre-mRNA. It interacts with the oncoprotein TLS, and abrogates the influence of TLS on E1A pre-mRNA splicing. This gene has multiple pseudogenes. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. In addition, transcript variants utilizing alternative polyA sites exist.

Uniprot Description

SRSF10: Splicing factor that in its dephosphorylated form acts as a general repressor of pre-mRNA splicing. Seems to interfere with the U1 snRNP 5'-splice recognition of SNRNP70. Required for splicing repression in M-phase cells and after heat shock. May be involved in regulation of alternative splicing in neurons, with isoform 1 acting as a positive and isoform 3 as a negative regulator. Belongs to the splicing factor SR family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; RNA splicing

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: nucleoplasm; cytoplasm; nuclear speck; nucleus

Molecular Function: protein binding; RS domain binding; RNA binding; unfolded protein binding; nucleotide binding

Biological Process: assembly of spliceosomal tri-snRNP; RNA splicing, via transesterification reactions; nuclear mRNA splicing, via spliceosome; mRNA splice site selection; mRNA export from nucleus; negative regulation of nuclear mRNA splicing, via spliceosome; regulation of transcription, DNA-dependent; cytoplasmic transport